ArticleAnalytical chemistry2012
Microfluidic device for the selective chemical stimulation of neurons and characterization of peptide release with mass spectrometry.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Microfluidic co-culture system for synaptically segregated neural networks to explore astrocyte-driven neural pathology.Microsystems & nanoengineering · 2026Article
- Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry.Sensors (Basel, Switzerland) · 2024Review
- Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology.RSC chemical biology · 2021Review
- Identifying Candidate Biomarkers of Ionizing Radiation in Human Pulmonary Microvascular Lumens Using Microfluidics-A Pilot Study.Micromachines · 2021Article
- Automated microfluidic cell culture of stem cell derived dopaminergic neurons.Scientific reports · 2019Article
- Integrating Mass Spectrometry with Microphysiological Systems for Improved Neurochemical Studies.Microphysiological systems · 2018Article
- Low-Density Neuronal Cultures from Human Induced Pluripotent Stem Cells.Molecular neuropsychiatry · 2017Article
- Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
- A neuron-in-capillary platform for facile collection and mass spectrometric characterization of a secreted neuropeptide.Scientific reports · 2016Article
- Peptidomics and Secretomics of the Mammalian Peripheral Sensory-Motor System.Journal of the American Society for Mass Spectrometry · 2015Article
- Peptidomics for the discovery and characterization of neuropeptides and hormones.Trends in pharmacological sciences · 2015Review
- Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples.Biochimica et biophysica acta · 2015Review
- Development of a microfluidic "click chip" incorporating an immobilized Cu(I) catalyst.RSC advances · 2015Article
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Stimulation and release from neurons via a dual capillary collection device interfaced to mass spectrometry.The Analyst · 2013Article
- Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration.Journal of neural engineering · 2013Article
- Microfluidic systems for studying neurotransmitters and neurotransmission.Lab on a chip · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 4 countries.
Funding
Abstract
Neuropeptides are synthesized in and released from neurons and are involved in a wide range of physiological processes, including temperature homeostasis, learning, memory, and disease. When working with sparse neuronal networks, the ability to collect and characterize small sample volumes is important as neurons often release only a small proportion of their mass-limited content. Microfluidic systems are well suited for the study of neuropeptides. They offer the ability to control and manipulate the extracellular environment and small sample volumes, thereby reducing the dilution of peptides following release. We present an approach for the culture and stimulation of a neuronal network within a microfluidic device, subsequent collection of the released peptides, and their detection via mass spectrometry. The system employs microvalve-controlled stimulation channels to selectively stimulate a low-density neuronal culture, allowing us to determine the temporal onset of peptide release. Released peptides from the well-characterized, peptidergic bag cell neurons of Aplysia californica were collected and their temporal pattern of release was characterized with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We show a robust difference in the timing of release for chemical solutions containing elevated K(+) (7 ± 3 min), when compared to insulin (19 ± 7 min) (p < 0.000 01).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.