ArticleCellular and molecular bioengineering2011
Microtubule Tip Tracking and Tip Structures at the Nanometer Scale Using Digital Fluorescence Microscopy.
Article in Cellular and molecular bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- Estradiol pauses microtubule growth without increased incidence of catastrophe events.Communications biology · 2025Article
- The GTP-tubulin cap is not the determinant of microtubule end stability in cells.Molecular biology of the cell · 2024Article
- Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends.eLife · 2024Article
- Beyond the GTP-cap: Elucidating the molecular mechanisms of microtubule catastrophe.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Tracing and tracking filamentous structures across scales: A systematic review.Computational and structural biotechnology journal · 2023Review
- Dynamic instability of dendrite tips generates the highly branched morphologies of sensory neurons.Science advances · 2022Article
- Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- Molecular mechanisms underlying microtubule growth dynamics.Current biology : CB · 2021Review
- Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: A review.Computational and structural biotechnology journal · 2021Review
- Tau Avoids the GTP Cap at Growing Microtubule Plus-Ends.iScience · 2020Article
- Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules.Current biology : CB · 2020Article
- Non-enzymatic Activity of the α-Tubulin Acetyltransferase αTAT Limits Synaptic Bouton Growth in Neurons.Current biology : CB · 2020Article
- Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends.eLife · 2019Article
- MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules.Scientific reports · 2019Article
- Rapid and inefficient kinetics of sickle hemoglobin fiber growth.Science advances · 2019Article
- Structured illumination approaches for super-resolution in plant cells.Microscopy (Oxford, England) · 2019Review
- Automated profiling of growth cone heterogeneity defines relations between morphology and motility.The Journal of cell biology · 2019Article
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Microtubules (MTs) are central to fundamental cellular processes including mitosis, polarization, and axon extension. A key issue is to understand how MT-associated proteins and therapeutic drugs, such as the anticancer drug paclitaxel, control MT self-assembly. To facilitate this research, it would be helpful to have automated methods that track the tip of dynamically assembling MTs as observed via fluorescence microscopy. Through a combination of digital fluorescence imaging with MT modeling, model-convolution, and automated image analysis of live and fixed MTs, we developed a method for MT tip tracking that includes estimation of the measurement error. We found that the typical single-frame tip tracking accuracy of GFP-tubulin labeled MTs in living LLC-PK1α cells imaged with a standard widefield epifluorescence digital microscope system is ~36 nm, the equivalent of ~4.5 tubulin dimer layers. However, if the MT tips are blunt, the tip tracking accuracy can be as accurate as ~15 nm (~2 dimer layers). By fitting a Gaussian survival function to the MT tip intensity profiles, we also established that MTs within living cells are not all blunt, but instead exhibit highly variable tapered tip structures with a protofilament length standard deviation of ~180 nm. More generally, the tip tracking method can be extended to track the tips of any individual fluorescently labeled filament, and can estimate filament tip structures both in vivo and in vitro with single-frame accuracy on the nanoscale.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.