ArticleJournal of bacteriology2012
Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Synergistic Effect of the Mucosa-Friendly Agents Berberine and Tea Tree Oil on Mucosal Protection AgainstAntibiotics (Basel, Switzerland) · 2026Article
- Neisseria gonorrhoeae induces local secretion of IL-10 at the human cervix to promote colonization.The Journal of clinical investigation · 2024Article
- Genetic Manipulation of Neisseria gonorrhoeae and Commensal Neisseria Species.Current protocols · 2024Article
- A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation.BMC microbiology · 2024Article
- In Vitro Analysis of Matched Isolates from Localized and Disseminated Gonococcal Infections Suggests That Opa Expression Impacts Clinical Outcome.Pathogens (Basel, Switzerland) · 2022Article
- Gonococcal invasion into epithelial cells depends on both cell polarity and ezrin.PLoS pathogens · 2021Article
- Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages.PLoS pathogens · 2021Article
- Extraction of Membrane Components fromPharmaceutics · 2020Article
- Role of the Gonococcal Neisserial Heparin Binding Antigen in Microcolony Formation, and Serum Resistance and Adherence to Epithelial Cells.The Journal of infectious diseases · 2020Article
- Neisseria gonorrhoeae infects the heterogeneous epithelia of the human cervix using distinct mechanisms.PLoS pathogens · 2019Article
- Neisseria gonorrhoeae uses cellular proteins CXCL10 and IL8 to enhance HIV-1 transmission across cervical mucosa.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining.Journal of visualized experiments : JoVE · 2019Article
- Article
- Neisseria gonorrhoeae infects the human endocervix by activating non-muscle myosin II-mediated epithelial exfoliation.PLoS pathogens · 2017Article
- nagZ Triggers Gonococcal Biofilm Disassembly.Scientific reports · 2016Article
- Expression of Opacity Proteins Interferes with the Transmigration of Neisseria gonorrhoeae across Polarized Epithelial Cells.PloS one · 2015Article
- Article
- Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils.Journal of bacteriology · 2013Article
- Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR.Cellular microbiology · 2013Article
- Phase variation leads to the misidentification of a Neisseria gonorrhoeae virulence gene.PloS one · 2013Article
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Authors and funding
9 authors.
Funding
Abstract
To better understand the role of Opa in gonococcal infections, we created and characterized a derivative of MS11 (MS11Δopa) that had the coding sequence for all 11 Opa proteins deleted. The MS11Δopa bacterium lost the ability to bind to purified lipooligosaccharide (LOS). While nonpiliated MS11Δopa and nonpiliated Opa-expressing MS11 cells grew at the same rate, nonpiliated MS11Δopa cells rarely formed clumps of more than four bacteria when grown in broth with vigorous shaking. Using flow cytometry analysis, we demonstrated that MS11Δopa produced a homogeneous population of bacteria that failed to bind monoclonal antibody (MAb) 4B12, a MAb specific for Opa. Opa-expressing MS11 cells consisted of two predominant populations, where ∼85% bound MAb 4B12 to a significant level and the other population bound little if any MAb. Approximately 90% of bacteria isolated from a phenotypically Opa-negative colony (a colony that does not refract light) failed to bind MAb 4B12; the remaining 10% bound MAb to various degrees. Piliated MS11Δopa cells formed dispersed microcolonies on ME180 cells which were visually distinct from those of piliated Opa-expressing MS11 cells. When Opa expression was reintroduced into MS11Δopa, the adherence ability of the strain recovered to wild-type levels. These data indicate that Opa contributes to both bacterium-bacterium and bacterium-host cell interactions.
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