ArticleApplied and environmental microbiology2012
Biosynthesis of cis,cis-muconic acid and its aromatic precursors, catechol and protocatechuic acid, from renewable feedstocks by Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed.
- UsingACS ES&T engineering · 2026Article
- Enhancement Strategy for Protocatechuic Acid Production UsingInternational journal of molecular sciences · 2025Article
- Enhancing metabolic efficiency via novel constitutive promoters to produce protocatechuic acid in Escherichia coli.Applied microbiology and biotechnology · 2024Article
- An Eclectic Review on Dicarboxylic Acid Production Through Yeast Cell Factories and Its Industrial Prominence.Current microbiology · 2024Review
- Sustainable biosynthetic pathways to value-added bioproducts from hydroxycinnamic acids.Applied microbiology and biotechnology · 2023Review
- Different Recognition of Protein Features Depending on Deep Learning Models: A Case Study of Aromatic Decarboxylase UbiD.Biology · 2023Article
- Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms · 2023Review
- Artificial microbial consortia for bioproduction processes.Engineering in life sciences · 2023Review
- Valorization of Polyethylene Terephthalate to Muconic Acid by EngineeringInternational journal of molecular sciences · 2022Article
- ComparingProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparative Analysis of Catabolic and Anabolic Dehydroshikimate Dehydratases for 3,4-DHBA Production inMicroorganisms · 2022Article
- Characterization of Catechol-1,2-Dioxygenase (Acdo1p) FromFrontiers in microbiology · 2022Article
- Article
- Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-Chem catalysis · 2021Article
- Integrating continuous hypermutation with high-throughput screening for optimization of cis,cis-muconic acid production in yeast.Microbial biotechnology · 2021Article
- Production of Protocatechuic Acid frommBio · 2021Article
- In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain.Microbial cell factories · 2021Article
- Article
- Discovery and Functional Analysis of a Salicylic Acid Hydroxylase from Aspergillus niger.Applied and environmental microbiology · 2021Article
- Crystal structures of non-oxidative decarboxylases reveal a new mechanism of action with a catalytic dyad and structural twists.Scientific reports · 2021Article
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6 authors.
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Abstract
Adipic acid is a high-value compound used primarily as a precursor for the synthesis of nylon, coatings, and plastics. Today it is produced mainly in chemical processes from petrochemicals like benzene. Because of the strong environmental impact of the production processes and the dependence on fossil resources, biotechnological production processes would provide an interesting alternative. Here we describe the first engineered Saccharomyces cerevisiae strain expressing a heterologous biosynthetic pathway converting the intermediate 3-dehydroshikimate of the aromatic amino acid biosynthesis pathway via protocatechuic acid and catechol into cis,cis-muconic acid, which can be chemically dehydrogenated to adipic acid. The pathway consists of three heterologous microbial enzymes, 3-dehydroshikimate dehydratase, protocatechuic acid decarboxylase composed of three different subunits, and catechol 1,2-dioxygenase. For each heterologous reaction step, we analyzed several potential candidates for their expression and activity in yeast to compose a functional cis,cis-muconic acid synthesis pathway. Carbon flow into the heterologous pathway was optimized by increasing the flux through selected steps of the common aromatic amino acid biosynthesis pathway and by blocking the conversion of 3-dehydroshikimate into shikimate. The recombinant yeast cells finally produced about 1.56 mg/liter cis,cis-muconic acid.
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