ArticleApplied and environmental microbiology2012
Choline and N,N-dimethylethanolamine as direct substrates for methanogens.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Methanogenesis from tetramethylammonium and choline inFrontiers in microbiology · 2026Article
- Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost.mSystems · 2024Article
- Methylotrophic methanogenesis in the Archaeoglobi revealed by cultivation of Ca. Methanoglobus hypatiae from a Yellowstone hot spring.The ISME journal · 2024Article
- Methyl-Based Methanogenesis: an Ecological and Genomic Review.Microbiology and molecular biology reviews : MMBR · 2023Review
- Methanogen activity and microbial diversity in Gulf of Cádiz mud volcano sediments.Frontiers in microbiology · 2023Article
- Diverse methylotrophic methanogenic archaea cause high methane emissions from seagrass meadows.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea.mSystems · 2021Article
- New Insights into the Ecology and Physiology ofMicroorganisms · 2020Article
- Several ways one goal-methanogenesis from unconventional substrates.Applied microbiology and biotechnology · 2020Review
- Genome Sequences of Two Choline-Utilizing Methanogenic Archaea,Microbiology resource announcements · 2019Article
- Deltaproteobacteria (Pelobacter) and Methanococcoides are responsible for choline-dependent methanogenesis in a coastal saltmarsh sediment.The ISME journal · 2019Article
- Examination of the Glycine Betaine-Dependent Methylotrophic Methanogenesis Pathway: Insights Into Anaerobic Quaternary Amine Methylotrophy.Frontiers in microbiology · 2019Article
- Coupled laboratory and field investigations resolve microbial interactions that underpin persistence in hydraulically fractured shales.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Origin and fate of methane in the Eastern Tropical North Pacific oxygen minimum zone.The ISME journal · 2017Article
- Anaerobic microbial community response to methanogenic inhibitors 2-bromoethanesulfonate and propynoic acid.MicrobiologyOpen · 2016Article
- Archaeal community diversity and abundance changes along a natural salinity gradient in estuarine sediments.FEMS microbiology ecology · 2015Article
- Draft Genome Sequence of an Obligately Methylotrophic Methanogen, Methanococcoides methylutens, Isolated from Marine Sediment.Genome announcements · 2014Article
- Glycine betaine as a direct substrate for methanogens (Methanococcoides spp.).Applied and environmental microbiology · 2014Article
- Effects of Spartina alterniflora invasion on the communities of methanogens and sulfate-reducing bacteria in estuarine marsh sediments.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Choline (N,N,N-trimethylethanolamine), which is widely distributed in membrane lipids and is a component of sediment biota, has been shown to be utilized anaerobically by mixed prokaryote cultures to produce methane but not by pure cultures of methanogens. Here, we show that five recently isolated Methanococcoides strains from a range of sediments (Aarhus Bay, Denmark; Severn Estuary mudflats at Portishead, United Kingdom; Darwin Mud Volcano, Gulf of Cadiz; Napoli mud volcano, eastern Mediterranean) can directly utilize choline for methanogenesis producing ethanolamine, which is not further metabolized. Di- and monomethylethanolamine are metabolic intermediates that temporarily accumulate. Consistent with this, dimethylethanolamine was shown to be another new growth substrate, but monomethylethanolamine was not. The specific methanogen inhibitor 2-bromoethanesulfonate (BES) inhibited methane production from choline. When choline and trimethylamine are provided together, diauxic growth occurs, with trimethylamine being utilized first, and then after a lag (∼7 days) choline is metabolized. Three type strains of Methanococcoides (M. methylutens, M. burtonii, and M. alaskense), in contrast, did not utilize choline. However, two of them (M. methylutens and M. burtonii) did metabolize dimethylethanolamine. These results extend the known substrates that can be directly utilized by some methanogens, giving them the advantage that they would not be reliant on bacterial syntrophs for their substrate supply.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.