ArticleTransgenic research2013
Native-sized spider silk proteins synthesized in planta via intein-based multimerization.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Design and Heterologous Production of Bagworm Silk-Inspired Proteins inComputational and structural biotechnology journal · 2026Article
- Functional Biomaterials Derived from Protein Liquid-Liquid Phase Separation and Liquid-to-Solid Transition.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Scale up of fermentation of recombinant Escherichia coli for efficient production of spider drag silk protein MaSp1s and its dimers.Microbial cell factories · 2025Article
- Recombinant production of spider silk protein in Physcomitrella photobioreactors.Plant cell reports · 2025Article
- Disentangling the Web: An Interdisciplinary Review on the Potential and Feasibility of Spider Silk Bioproduction.ACS biomaterials science & engineering · 2024Review
- High mechanical property silk produced by transgenic silkworms expressing theFrontiers in bioengineering and biotechnology · 2024Article
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- Liquid-Liquid Phase Separation and Assembly of Silk-like Proteins is Dependent on the Polymer Length.Biomacromolecules · 2022Article
- Engineering Circularized mRNAs for the Production of Spider Silk Proteins.Applied and environmental microbiology · 2022Article
- Recombinant Spider Silk: Promises and Bottlenecks.Frontiers in bioengineering and biotechnology · 2022Review
- Advances in Plant-Derived Scaffold Proteins.Frontiers in plant science · 2020Review
- Synthetic biology for fibres, adhesives and active camouflage materials in protection and aerospace.MRS communications · 2019Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- A Facile Measurement for Monitoring Dragline Silk Dope Concentration inMaterials (Basel, Switzerland) · 2018Article
- Mass spider silk production through targeted gene replacement inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Method for the Destruction of Endotoxin in Synthetic Spider Silk Proteins.Scientific reports · 2018Article
- Spider silk-like proteins derived from transgenic Nicotiana tabacum.Transgenic research · 2016Article
- Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability.Frontiers in plant science · 2016Article
- In planta production of ELPylated spidroin-based proteins results in non-cytotoxic biopolymers.BMC biotechnology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthesis of native-sized proteins is a pre-requisite for exploiting the potential of spider silk as a bio-based material. The unique properties of spider silk, such as extraordinary tensile strength and elasticity, result from the highly repetitive nature of spider silk protein motifs. The present report describes the combination of spider silk flagelliform protein (FLAG) production in the endoplasmic reticulum of tobacco plant leaf cells with an intein-based posttranslational protein fusion technology. The repeated ligation of FLAG monomers resulted in the formation of large multimers. This method avoids the need for highly repetitive transgenes, which may result in a higher genetic and transcriptional stability. Here we show, for the first time, the production of synthetic, high molecular weight spider silk proteins larger than 250 kDa based on the assembly of protein monomers via intein-mediated trans-splicing in planta. The resulting multimeric structures form microfibers, thereby demonstrating their great potential as a biomaterial.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.