ReviewAnalytical and bioanalytical chemistry2013
Application of quantitative PCR for the detection of microorganisms in water.
Review in Analytical and bioanalytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Comparative evaluation of two high-throughput qPCR systems for environmental and clinical microbiology applications.Applied and environmental microbiology · 2026Article
- Digital PCR Reveals High PvDBP1 but not PvEBP/DBP2 and PvRBP2b Copy Number in Plasmodium vivax From Duffy-Negative Individuals in Central Africa.The Journal of infectious diseases · 2026Article
- Comparison of ESKAPE Pathogen Levels in Wastewater and Receiving Water Bodies Using Agar-Based Enumeration and Real-Time PCR.Current microbiology · 2026Article
- Reactive Quantum Dot-Based FRET Systems for Target-Catalyzed Detection of RNA.Methods in molecular biology (Clifton, N.J.) · 2026Article
- QMRA for assessing treatment needs of surface water for drinking: trends and challenges in fecal pathogen quantification.Journal of water and health · 2025Review
- Optimization ofJournal of environmental engineering (New York, N.Y.) · 2025Article
- Spatiotemporal Analysis of the Distribution of Waterborne Diseases in Children Under 5 Years of Age From 2018 to 2022 in the Lemba Health Zone in Kinshasa, DR Congo: A Retrospective and Observational Analysis.Health science reports · 2025Article
- Development and evaluation of fluorescent recombinase polymerase amplification (RPA)-based method for rapid detection of Necator americanus.PLoS neglected tropical diseases · 2025Article
- Article
- Droplet Digital PCR Provides Highly Sensitive and Accurate Opsin Gene SNP Detection From Wild Primate Fecal Samples.Ecology and evolution · 2025Article
- Enhanced Detection of ViableMicroorganisms · 2024Article
- qPCR-based phytoplankton abundance and chlorophyll a: A multi-year study in twelve large freshwater rivers across the United States.The Science of the total environment · 2024Article
- The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater.Scientific reports · 2024Article
- Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings.Diagnostics (Basel, Switzerland) · 2024Review
- Next-generation microbiological testing in intraabdominal infections with PCR technology.Langenbeck's archives of surgery · 2024Article
- Micromonas, a small pigmented flagellate, predominates the nanoflagellate and photosynthetic picoeukaryote communities in the northern South China Sea.Environmental microbiology reports · 2024Article
- Rapid detection method of bacterial pathogens in surface waters and a new risk indicator for water pathogenic pollution.Scientific reports · 2024Article
- Performance evaluation of a low-throughput qPCR-based Legionella assay for utility as an onsite industrial water system monitoring method.Journal of industrial microbiology & biotechnology · 2024Article
- Review
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The occurrence of microorganisms in water due to contamination is a health risk and control thereof is a necessity. Conventional detection methods may be misleading and do not provide rapid results allowing for immediate action. The quantitative polymerase chain reaction (qPCR) method has proven to be an effective tool to detect and quantify microorganisms in water within a few hours. Quantitative PCR assays have recently been developed for the detection of specific adeno- and polyomaviruses, bacteria and protozoa in different water sources. The technique is highly sensitive and able to detect low numbers of microorganisms. Quantitative PCR can be applied for microbial source tracking in water sources, to determine the efficiency of water and wastewater treatment plants and act as a tool for risk assessment. Different qPCR assays exist depending on whether an internal control is used or whether measurements are taken at the end of the PCR reaction (end-point qPCR) or in the exponential phase (real-time qPCR). Fluorescent probes are used in the PCR reaction to hybridise within the target sequence to generate a signal and, together with specialised systems, quantify the amount of PCR product. Quantitative reverse transcription polymerase chain reaction (q-RT-PCR) is a more sensitive technique that detects low copy number RNA and can be applied to detect, e.g. enteric viruses and viable microorganisms in water, and measure specific gene expression. There is, however, a need to standardise qPCR protocols if this technique is to be used as an analytical diagnostic tool for routine monitoring. This review focuses on the application of qPCR in the detection of microorganisms in water.
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