ArticleMolecular and cellular biology1990
A combination of closely associated positive and negative cis-acting promoter elements regulates transcription of the skeletal alpha-actin gene.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- Cardiac gene activation analysis in mammalian non-myoblasic cells by Nkx2-5, Tbx5, Gata4 and Myocd.PloS one · 2012Article
- MicroRNA genes preferentially expressed in dendritic cells contain sites for conserved transcription factor binding motifs in their promoters.BMC genomics · 2011Article
- Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation.Genome biology · 2007Article
- Serum response factor MADS box serine-162 phosphorylation switches proliferation and myogenic gene programs.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway.Nucleic acids research · 2005Article
- Increased level and duration of expression in muscle by co-expression of a transactivator using plasmid systems.Gene therapy · 1999Article
- Activation of the skeletal alpha-actin promoter during muscle regeneration.Journal of muscle research and cell motility · 1998Article
- Influence of promoter potency on the transcriptional effects of YY1, SRF and Msx-1 in transient transfection analysis.Nucleic acids research · 1998Article
- The Rho family G proteins play a critical role in muscle differentiation.Molecular and cellular biology · 1998Article
- Cell-specific transcription of the smooth muscle gamma-actin gene requires both positive- and negative-acting cis elements.Gene expression · 1998Article
- Recruitment of the tinman homolog Nkx-2.5 by serum response factor activates cardiac alpha-actin gene transcription.Molecular and cellular biology · 1996Article
- Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle.Molecular and cellular biology · 1995Article
- A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene.Molecular and cellular biology · 1995Article
- Regulation of a muscle-specific transgene by retinoic acid.The Journal of cell biology · 1995Article
- Identification of a DNA binding site for the nuclear factor YY1 in the human GM-CSF core promoter.Nucleic acids research · 1994Article
- Transcriptional control of the chicken cardiac myosin light-chain gene is mediated by two AT-rich cis-acting DNA elements and binding of serum response factor.Molecular and cellular biology · 1993Article
- Multiple regulatory elements contribute differentially to muscle creatine kinase enhancer activity in skeletal and cardiac muscle.Molecular and cellular biology · 1993Article
- Regulation of expression of transgenes in developing fish.Transgenic research · 1993Article
- Tissue-specific restriction of skeletal muscle troponin C gene expression.Gene expression · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The chicken skeletal alpha-actin gene promoter region provides at least a 75-fold-greater transcriptional activity in muscle cells than in fibroblasts. The cis-acting sequences required for cell type-restricted expression within this 200-base-pair (bp) region were elucidated by chloramphenicol acetyltransferase assays of site-directed Bg/II linker-scanning mutations transiently transfected into primary cultures. Four positive cis-acting elements were identified and are required for efficient transcriptional activity in myogenic cells. These elements, conserved across vertebrate evolution, include the ATAAAA box (-24 bp), paired CCAAT-box-associated repeats (CBARs; at -83 bp and -127 bp), and the upstream T+A-rich regulatory sequence (at -176 bp). Basal transcriptional activity in fibroblasts was not as dependent on the upstream CBAR or regions of the upstream T+A-rich regulatory sequence. Transfection experiments provided evidence that positive regulatory factors required for alpha-actin expression in fibroblasts are limiting. In addition, negative cis-acting elements were detected and found closely associated with the G+C-rich sequences that surround the paired CBARs. Negative elements may have a role in restricting developmentally timed expression in myoblasts and appear to inhibit promoter activity in nonmyogenic cells. Cell type-specific expression of the skeletal alpha-actin gene promoter is regulated by combinatorial and possibly competitive interactions between multiple positive and negative cis-acting elements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.