ArticleBiochemical and biophysical research communications2012
Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells.
Article in Biochemical and biophysical research communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- The Synthetic Cannabinoid ADB-FUBINACA Disrupts Mitochondrial Morphology and Dynamics during Neuronal Differentiation of NG108-15 Cells.Molecular neurobiology · 2026Article
- Ngn2-Induced Differentiation of the NG108-15 Cell Line Enhances Motor Neuronal Differentiation and Neuromuscular Junction Formation.Biomolecules · 2025Article
- Recent Developments in Botulinum Neurotoxins Detection.Microorganisms · 2022Review
- The Synthetic Cannabinoids THJ-2201 and 5F-PB22 Enhance In Vitro CBInternational journal of molecular sciences · 2020Article
- Development of a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A through Neural Culture Media Optimization.Journal of biomolecular screening · 2016Article
- Recent developments in cell-based assays and stem cell technologies for botulinum neurotoxin research and drug discovery.Expert review of molecular diagnostics · 2014Review
- Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro.Infection and immunity · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
Cancerous cell lines have traditionally shown low sensitivity to laboratory or pharmaceutical preparations of botulinum neurotoxin. The work presented here demonstrates that the mouse neuroblastoma/rat glioma hybrid cell line NG108-15 is capable of more sensitively detecting BoNT/A1 than any cell line previously described. This cell line has previously been described to have motor neuron like characteristics, therefore making it a good model to study BoNTs. Differentiation of NG108-15 cells in serum-free medium containing retinoic acid and purmorphamine dramatically increased sensitivity of the neurons to BoNT/A (EC(50) = ~16 LD(50) U). Additional pre-treatment with triasialoganglioside GT1B prior to toxin exposure reduced the EC(50) further to ~11 LD(50) U. Co-culture of the neurons with C2C12 myotubes also significantly increased BoNT/A sensitivity of NG108-15 cells (EC(50) = 26 U) in the absence of differentiation factors.
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