ArticleCell reports2012
High-throughput screening for bioactive molecules using primary cell culture of transgenic zebrafish embryos.
Article in Cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Review
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- Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology.Marine biotechnology (New York, N.Y.) · 2023Review
- Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish.The Journal of veterinary medical science · 2021Article
- Article
- From fish bowl to bedside: The power of zebrafish to unravel melanoma pathogenesis and discover new therapeutics.Pigment cell & melanoma research · 2017Review
- A glycan-based approach to therapeutic angiogenesis.PloS one · 2017Article
- Directed Differentiation of Zebrafish Pluripotent Embryonic Cells to Functional Cardiomyocytes.Stem cell reports · 2016Article
- Lanthanide Hydroxide Nanoparticles Induce Angiogenesis via ROS-Sensitive Signaling.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock.eLife · 2016Article
- Embryonic cell culture in zebrafish.Methods in cell biology · 2016Article
- Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: focus on the cancer hallmark of tumor angiogenesis.Carcinogenesis · 2015Review
- Current approaches for the discovery of drugs that deter substance and drug abuse.Expert opinion on drug discovery · 2014Review
- Regenerative medicine: Of fish and men.Nature chemical biology · 2014Article
- A zebrafish embryo culture system defines factors that promote vertebrate myogenesis across species.Cell · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Transgenic zebrafish embryos expressing tissue-specific green fluorescent protein (GFP) can provide an unlimited supply of primary embryonic cells. Agents that promote the differentiation of these cells may be beneficial for therapeutics. We report a high-throughput approach for screening small molecules that regulate cell differentiation using lineage-specific GFP transgenic zebrafish embryonic cells. After validating several known regulators of the differentiation of endothelial and other cell types, we performed a screen for proangiogenic molecules using undifferentiated primary cells from flk1-GFP transgenic zebrafish embryos. Cells were grown in 384-well plates with 12,128 individual small molecules, and GFP expression was analyzed by means of an automated imaging system, which allowed us to screen thousands of compounds weekly. As a result, 23 molecules were confirmed to enhance angiogenesis, and 11 of them were validated to promote the proliferation of mammalian human umbilical vascular endothelial cells and induce Flk1+ cells from murine embryonic stem cells. We demonstrated the general applicability of this strategy by analyzing additional cell lineages using zebrafish expressing GFP in pancreatic, cardiac, and dopaminergic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.