ArticleBiomaterials2012
Influence of a three-dimensional, microarray environment on human cell culture in drug screening systems.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Cell-embedded microgels as emerging miniature 3D tissue-mimics toward biochip-based toxicity screening.Bioengineering & translational medicine · 2026Review
- A Review on New Frontiers in Drug-Drug Interaction Predictions and Safety Evaluations with In Vitro Cellular Models.Pharmaceutics · 2025Review
- A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening.Scientific reports · 2025Article
- Biodegradable Gelatin-Carrageenan Sponges: High-Potential Functional Nasal Packs for Efficient Secretome Delivery.Polymers · 2024Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Innovative explorations: unveiling the potential of organoids for investigating environmental pollutant exposure.Environmental science and pollution research international · 2024Review
- The population context is a driver of the heterogeneous response of epithelial cells to interferons.Molecular systems biology · 2024Article
- Three-Dimensional In Vitro Cell Culture Models for Efficient Drug Discovery: Progress So Far and Future Prospects.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Recent Developments in Artificial Super-Wettable Surfaces Based on Bioinspired Polymeric Materials for Biomedical Applications.Polymers · 2022Review
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Drug Evaluation Based on a Multi-Channel Cell Chip with a Horizontal Co-Culture.International journal of molecular sciences · 2021Article
- Article
- Biological features, gene expression profile, and mechanisms of drug resistance of two- and three-dimensional hepatocellular carcinoma cell cultures.Pharmacology research & perspectives · 2021Article
- Article
- Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.Stem cells and development · 2019Article
- Next-Generation Liver Medicine Using Organoid Models.Frontiers in cell and developmental biology · 2019Review
- High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture.Biotechnology and bioengineering · 2019Article
- β1 integrin-mediated multicellular resistance in hepatocellular carcinoma through activation of the FAK/Akt pathway.The Journal of international medical research · 2018Article
- Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We have used a modified 3D cellular microarray platform for the high-throughput analysis of growth, cytotoxicity, and protein expression profile of a human hepatocellular carcinoma cell line, HepG2, in alginate. The results obtained were compared to analogous studies in 2D and 3D environments at the microtiter scale. The antiproliferative effects of four drugs, tamoxifen, 5-fluorouracil, doxorubicin, and amitriptyline, were studied as a function of seeding density in the three different culture platforms. The chemosensitivity of HepG2 cells to all four compounds decreased substantially with increasing cell number in the 2D and 3D microtiter-based cultures, while no seeding density dependence was observed in the IC(50) values obtained in the 3D microarray culture platform. These results can be rationalized based on the development of confluence-dependent resistance in cultures where proliferation is restricted by cell-cell contacts and nutrient availability, as is the case for both of the microtiter-based cultures. Additionally, further development of an on-chip, in-cell immunofluorescence assay provided quantitative data on the levels of specific target proteins involved in proliferation, adhesion, angiogenesis and drug metabolism, and was used to compare expression profiles between 2D and 3D environments. The up-regulation of several CYP450 enzymes, β1-integrin and vascular endothelial growth factor (VEGF) in the 3D microarray cultures suggests that this platform provides a more in vivo-like environment allowing cells to approach their natural phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.