ArticleBiochemistry2012
Lack of Csk-mediated negative regulation in a unicellular SRC kinase.
Article in Biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- An evolutionary and physiological perspective on cell-substrate adhesion machinery for cell migration.Frontiers in cell and developmental biology · 2022Review
- The origin of animals: an ancestral reconstruction of the unicellular-to-multicellular transition.Open biology · 2021Review
- Structural characterization and computational analysis of PDZ domains in Monosiga brevicollis.Protein science : a publication of the Protein Society · 2020Article
- Temperature sensitivities of metazoan and pre-metazoan Src kinases.Biochemistry and biophysics reports · 2020Article
- The Src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases.Critical reviews in biochemistry and molecular biology · 2018Review
- Transfection ofDevelopment (Cambridge, England) · 2018Article
- Src signaling in a low-complexity unicellular kinome.Scientific reports · 2018Article
- A switch in nucleotide affinity governs activation of the Src and Tec family kinases.Scientific reports · 2017Article
- Early emergence of negative regulation of the tyrosine kinase Src by the C-terminal Src kinase.The Journal of biological chemistry · 2017Article
- Phosphotyrosine signalling and the origin of animal multicellularity.Proceedings. Biological sciences · 2017Article
- Auto-thiophosphorylation activity of Src tyrosine kinase.BMC biochemistry · 2016Article
- Article
- Exploring the evolutionary history of centrosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Endocytosis and the Src family of non-receptor tyrosine kinases.Biomolecular concepts · 2014Review
- Regulation of Src and Csk nonreceptor tyrosine kinases in the filasterean Ministeria vibrans.Biochemistry · 2014Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Phosphotyrosine-based signaling plays a vital role in cellular communication in multicellular organisms. Unexpectedly, unicellular choanoflagellates (the closest phylogenetic group to metazoans) possess numbers of tyrosine kinases that are comparable to those in complex metazoans. Here, we have characterized tyrosine kinases from the filasterean Capsaspora owczarzaki, a unicellular protist representing the sister group to choanoflagellates and metazoans. Two Src-like tyrosine kinases have been identified in C. owczarzaki (CoSrc1 and CoSrc2), both of which have the arrangement of SH3, SH2, and catalytic domains seen in mammalian Src kinases. In Capsaspora cells, CoSrc1 and CoSrc2 localize to punctate structures in filopodia that may represent primordial focal adhesions. We have cloned, expressed, and purified both enzymes. CoSrc1 and CoSrc2 are active tyrosine kinases. Mammalian Src kinases are normally regulated in a reciprocal fashion by autophosphorylation in the activation loop (which increases activity) and by Csk-mediated phosphorylation of the C-terminal tail (which inhibits activity). Similar to mammalian Src kinases, the enzymatic activities of CoSrc1 and CoSrc2 are increased by autophosphorylation in the activation loop. We have identified a Csk-like kinase (CoCsk) in the genome of C. owczarzaki. We cloned, expressed, and purified CoCsk and found that it has no measurable tyrosine kinase activity. Furthermore, CoCsk does not phosphorylate or regulate CoSrc1 or CoSrc2 in cells or in vitro, and CoSrc1 and CoSrc2 are active in Capsaspora cell lysates. Thus, the function of Csk as a negative regulator of Src family kinases appears to have arisen with the emergence of metazoans.
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