ArticleNeurogastroenterology and motility2013
Nestin-expressing cells in the gut give rise to enteric neurons and glial cells.
Article in Neurogastroenterology and motility, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 57 citations in OpenAlex.
- Inflammation rewires the enteric nervous system through neurogenic monocyte recruitment.The Journal of experimental medicine · 2026Article
- T cells regulate intestinal motility and shape enteric neuronal responses to intestinal microbiota.Gut microbes · 2025Article
- TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome.NPJ biofilms and microbiomes · 2025Article
- The Role ofmedRxiv : the preprint server for health sciences · 2025Article
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- Harnessing the Power of Enteric Glial Cells' Plasticity and Multipotency for Advancing Regenerative Medicine.International journal of molecular sciences · 2023Review
- The enteric nervous system.Physiological reviews · 2023Review
- Prucalopride might improve intestinal motility by promoting the regeneration of the enteric nervous system in diabetic rats.International journal of molecular medicine · 2022Article
- Reply.Cellular and molecular gastroenterology and hepatology · 2022Article
- Intestinal microbiota shapes gut physiology and regulates enteric neurons and glia.Microbiome · 2021Article
- The Interplay between Nutrition, Innate Immunity, and the Commensal Microbiota in Adaptive Intestinal Morphogenesis.Nutrients · 2021Review
- Disorders of the enteric nervous system - a holistic view.Nature reviews. Gastroenterology & hepatology · 2021Review
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- Rett syndrome: think outside the (skull) box.Faculty reviews · 2021Review
- Development, Diversity, and Neurogenic Capacity of Enteric Glia.Frontiers in cell and developmental biology · 2021Review
- Transgenic models for investigating the nervous system: Currently available neurofluorescent reporters and potential neuronal markers.Biochimica et biophysica acta. General subjects · 2020Review
- Article
- Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain.Frontiers in physiology · 2019Article
- Physiological functions of ferroportin in the regulation of renal iron recycling and ischemic acute kidney injury.American journal of physiology. Renal physiology · 2018Article
- Gut microbiota regulates maturation of the adult enteric nervous system via enteric serotonin networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundNeuronal stem cells (NSCs) are promising for neurointestinal disease therapy. Although NSCs have been isolated from intestinal musclularis, their presence in mucosa has not been well described. Mucosa-derived NSCs are accessible endoscopically and could be used autologously. Brain-derived Nestin-positive NSCs are important in endogenous repair and plasticity. The aim was to isolate and characterize mucosa-derived NSCs, determine their relationship to Nestin-expressing cells and to demonstrate their capacity to produce neuroglial networks in vitro and in vivo.
methodsNeurospheres were generated from periventricular brain, colonic muscularis (Musc), and mucosa-submucosa (MSM) of mice expressing green fluorescent protein (GFP) controlled by the Nestin promoter (Nestin-GFP). Neuronal stem cells were also grown as adherent colonies from intestinal mucosal organoids. Their differentiation potential was assessed using immunohistochemistry using glial and neuronal markers. Brain and gut-derived neurospheres were transplanted into explants of chick embryonic aneural hindgut to determine their fate. KEY
resultsMusc- and MSM-derived neurospheres expressed Nestin and gave rise to cells of neuronal, glial, and mesenchymal lineage. Although Nestin expression in tissue was mostly limited to glia co-labelled with glial fibrillary acid protein (GFAP), neurosphere-derived neurons and glia both expressed Nestin in vitro, suggesting that Nestin+/GFAP+ glial cells may give rise to new neurons. Moreover, following transplantation into aneural colon, brain- and gut-derived NSCs were able to differentiate into neurons. CONCLUSIONS & INFERENCES: Nestin-expressing intestinal NSCs cells give rise to neurospheres, differentiate into neuronal, glial, and mesenchymal lineages in vitro, generate neurons in vivo and can be isolated from mucosa. Further studies are needed for exploring their potential for treating neuropathies.
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