ArticleBlood2012
Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy.
Article in Blood, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Clonal tracking in cancer and metastasis.Cancer metastasis reviews · 2024Review
- In vivo observation of multi-phase spatiotemporal cellular dynamics of transplanted HSPCs during early engraftment.FASEB bioAdvances · 2022Article
- Clonal tracking of haematopoietic cells: insights and clinical implications.British journal of haematology · 2021Review
- Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation.Stem cell research & therapy · 2020Article
- Review
- Tissue-Resident PDGFRαCell reports · 2020Article
- Combining near-infrared fluorescence with Brainbow to visualize expression of specific genes within a multicolor context.Molecular biology of the cell · 2019Article
- Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation.The Journal of experimental medicine · 2018Article
- Lentiviral Fluorescent Genetic Barcoding for Multiplex Fate Tracking of Leukemic Cells.Molecular therapy. Methods & clinical development · 2017Article
- Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior.Advanced drug delivery reviews · 2017Review
- Article
- Endosome-mitochondria interactions are modulated by iron release from transferrin.The Journal of cell biology · 2016Article
- Defining Clonal Color in Fluorescent Multi-Clonal Tracking.Scientific reports · 2016Article
- Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models.BoneKEy reports · 2016Article
- Functional Niche Competition Between Normal Hematopoietic Stem and Progenitor Cells and Myeloid Leukemia Cells.Stem cells (Dayton, Ohio) · 2015Article
- Bone marrow-derived mesenchymal stromal cells harness purinergenic signaling to tolerize human Th1 cells in vivo.Stem cells (Dayton, Ohio) · 2015Article
- Brainbow: new resources and emerging biological applications for multicolor genetic labeling and analysis.Genetics · 2015Review
- In-vivo RGB marking and multicolour single-cell tracking in the adult brain.Scientific reports · 2014Article
- Single-cell technologies sharpen up mammalian stem cell research.Nature cell biology · 2014Review
- In vivo clonal tracking of hematopoietic stem and progenitor cells marked by five fluorescent proteins using confocal and multiphoton microscopy.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We demonstrate a methodology for tracing the clonal history of hematopoietic stem and progenitor cells (HSPCs) behavior in live tissues in 4 dimensions (4D). This integrates genetic combinatorial marking using lentiviral vectors encoding various fluorescent proteins (FPs) with advanced imaging methods. Five FPs: Cerulean, EGFP, Venus, tdTomato, and mCherry were concurrently used to create a diverse palette of color-marked cells. A key advantage of imaging using a confocal/2-photon hybrid microscopy approach is the simultaneous assessment of uniquely 5FP-marked cells in conjunction with structural components of the tissues at high resolution. Volumetric analyses revealed that spectrally coded HSPC-derived cells can be detected noninvasively in various intact tissues, including the bone marrow, for extensive periods of time after transplantation. Live studies combining video-rate multiphoton and confocal imaging in 4D demonstrate the possibility of dynamic cellular and clonal tracking in a quantitative manner. This methodology has applications in the understanding of clonal architecture in normal and perturbed hematopoiesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.