ArticleRNA biology2012
Specific temperature-induced perturbations of secondary mRNA structures are associated with the cold-adapted temperature-sensitive phenotype of influenza A virus.
Article in RNA biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Advances and challenges in avian influenza vaccines: From conventional approaches to next-generation strategies.Veterinary and animal science · 2026Review
- Temperature dependent viral tropism: understanding viral seasonality and pathogenicity as applied to the avoidance and treatment of endemic viral respiratory illnesses.Reviews in medical virology · 2022Review
- Group II intron as cold sensor for self-preservation and bacterial conjugation.Nucleic acids research · 2020Article
- Changes in RNA secondary structure affect NS1 protein expression during early stage influenza virus infection.Virology journal · 2019Article
- Active 5' splice sites regulate the biogenesis efficiency of Arabidopsis microRNAs derived from intron-containing genes.Nucleic acids research · 2017Article
- Computational and molecular analysis of conserved influenza A virus RNA secondary structures involved in infectious virion production.RNA biology · 2016Article
- The influenza A virus NS genome segment displays lineage-specific patterns in predicted RNA secondary structure.BMC research notes · 2016Article
- Seasonality and selective trends in viral acute respiratory tract infections.Medical hypotheses · 2016Article
- Prediction of the next highly pathogenic avian influenza pandemic that can cause illness in humans.Infectious diseases of poverty · 2015Article
- RNA structural constraints in the evolution of the influenza A virus genome NP segment.RNA biology · 2014Article
- RNAtips: Analysis of temperature-induced changes of RNA secondary structure.Nucleic acids research · 2013Article
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Authors and funding
7 authors.
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Abstract
For decades, cold-adapted, temperature-sensitive (ca/ts) strains of influenza A virus have been used as live attenuated vaccines. Due to their great public health importance it is crucial to understand the molecular mechanism(s) of cold adaptation and temperature sensitivity that are currently unknown. For instance, secondary RNA structures play important roles in influenza biology. Thus, we hypothesized that a relatively minor change in temperature (32-39°C) can lead to perturbations in influenza RNA structures and, that these structural perturbations may be different for mRNAs of the wild type (wt) and ca/ts strains. To test this hypothesis, we developed a novel in silico method that enables assessing whether two related RNA molecules would undergo (dis)similar structural perturbations upon temperature change. The proposed method allows identifying those areas within an RNA chain where dissimilarities of RNA secondary structures at two different temperatures are particularly pronounced, without knowing particular RNA shapes at either temperature. We identified such areas in the NS2, PA, PB2 and NP mRNAs. However, these areas are not identical for the wt and ca/ts mutants. Differences in temperature-induced structural changes of wt and ca/ts mRNA structures may constitute a yet unappreciated molecular mechanism of the cold adaptation/temperature sensitivity phenomena.
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