ArticleBiophysical journal2012
Mapping of bacterial biofilm local mechanics by magnetic microparticle actuation.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Mechanical Comparison ofACS biomaterials science & engineering · 2025Article
- Alginate exopolymer significantly modulates the viscoelastic properties and resilience of bacterial biofilms.NPJ biofilms and microbiomes · 2025Article
- Adaptations of Gram-Negative and Gram-Positive Probiotic Bacteria in Engineered Living Materials.ACS biomaterials science & engineering · 2025Article
- Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges.Microbiology and molecular biology reviews : MMBR · 2025Review
- Article
- Effect of biofilm physical characteristics on their susceptibility to antibiotics: impacts of low-frequency ultrasound.NPJ biofilms and microbiomes · 2024Article
- Disturbing the Spatial Organization of Biofilm Communities Affects Expression ofApplied and environmental microbiology · 2023Article
- In Vitro Models of Bacterial Biofilms: Innovative Tools to Improve Understanding and Treatment of Infections.Nanomaterials (Basel, Switzerland) · 2023Review
- Dynamic Changes in Biofilm Structures under Dynamic Flow Conditions.Applied and environmental microbiology · 2022Article
- Nanotubes Formation inCells · 2022Article
- Dependency of hydration and growth conditions on the mechanical properties of oral biofilms.Scientific reports · 2021Article
- Arginine InducedFrontiers in cellular and infection microbiology · 2021Article
- Biofilm and swarming emergent behaviours controlled through the aid of biophysical understanding and tools.Biochemical Society transactions · 2020Review
- Review
- Bacterial biomechanics-From individual behaviors to biofilm and the gut flora.APL bioengineering · 2020Review
- Development of a New Bead Movement-Based Computational Framework Shows that Bacterial Amyloid Curli Reduces Bead Mobility in Biofilms.Journal of bacteriology · 2020Article
- Generation of High Dose Inhalable Effervescent Dispersions against Pseudomonas aeruginosa Biofilms.Pharmaceutical research · 2020Article
- Conformable self-assembling amyloid protein coatings with genetically programmable functionality.Science advances · 2020Article
- Regulating, Measuring, and Modeling the Viscoelasticity of Bacterial BiofilmsJournal of bacteriology · 2019Review
- Testing Anti-Biofilm Polymeric Surfaces: Where to Start?International journal of molecular sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most bacteria live in the form of adherent communities forming three-dimensional material anchored to artificial or biological surfaces, with profound impact on many human activities. Biofilms are recognized as complex systems but their physical properties have been mainly studied from a macroscopic perspective. To determine biofilm local mechanical properties, reveal their potential heterogeneity, and investigate their relation to molecular traits, we have developed a seemingly new microrheology approach based on magnetic particle infiltration in growing biofilms. Using magnetic tweezers, we achieved what was, to our knowledge, the first three-dimensional mapping of the viscoelastic parameters on biofilms formed by the bacterium Escherichia coli. We demonstrate that its mechanical profile may exhibit elastic compliance values spread over three orders of magnitude in a given biofilm. We also prove that heterogeneity strongly depends on external conditions such as growth shear stress. Using strains genetically engineered to produce well-characterized cell surface adhesins, we show that the mechanical profile of biofilm is exquisitely sensitive to the expression of different surface appendages such as F pilus or curli. These results provide a quantitative view of local mechanical properties within intact biofilms and open up an additional avenue for elucidating the emergence and fate of the different microenvironments within these living materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.