ArticleBiophysical journal2012
Thick-to-thin filament surface distance modulates cross-bridge kinetics in Drosophila flight muscle.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The E525K β-Myosin Mutation Causes Hypocontractility in Cardiomyocytes Without Altering Loaded Crossbridge Cycling.bioRxiv : the preprint server for biology · 2026Article
- A primer on the methods of skeletal and cardiac muscle mechanics using permeabilized preparations.The Journal of general physiology · 2026Review
- Developmental remodelling ofOpen biology · 2025Article
- Bayesian estimation of muscle mechanisms and therapeutic targets using variational autoencoders.Biophysical journal · 2025Article
- Bayesian Estimation of Muscle Mechanisms and Therapeutic Targets Using Variational Autoencoders.bioRxiv : the preprint server for biology · 2024Article
- The structural and functional effects of myosin regulatory light chain phosphorylation are amplified by increases in sarcomere length and [CaThe Journal of physiology · 2024Article
- Disruption of Z-Disc Function Promotes Mechanical Dysfunction in Human Myocardium: Evidence for a Dual Myofilament Modulatory Role by Alpha-Actinin 2.International journal of molecular sciences · 2023Article
- Cardiac troponin T N-domain variant destabilizes the actin interface resulting in disturbed myofilament function.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nanometer-scale structure differences in the myofilament lattice spacing of two cockroach leg muscles correspond to their different functions.The Journal of experimental biology · 2020Article
- Structural and functional impact of troponin C-mediated CaJournal of molecular and cellular cardiology · 2018Article
- Altered myofilament structure and function in dogs with Duchenne muscular dystrophy cardiomyopathy.Journal of molecular and cellular cardiology · 2018Article
- Multiscale and Multiaxial Mechanics of Vascular Smooth Muscle.Biophysical journal · 2017Article
- Myosin MgADP Release Rate Decreases as Sarcomere Length Increases in Skinned Rat Soleus Muscle Fibers.Biophysical journal · 2016Article
- Mechano-chemical Interactions in Cardiac Sarcomere Contraction: A Computational Modeling Study.PLoS computational biology · 2016Article
- Increased Titin Compliance Reduced Length-Dependent Contraction and Slowed Cross-Bridge Kinetics in Skinned Myocardial Strips from Rbm (20ΔRRM) Mice.Frontiers in physiology · 2016Article
- Kinetics of cardiac myosin isoforms in mouse myocardium are affected differently by presence of myosin binding protein-C.Journal of muscle research and cell motility · 2014Article
- The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils.The Journal of cell biology · 2014Article
- Myofilament lattice structure in presence of a skeletal myopathy-related tropomyosin mutation.Journal of muscle research and cell motility · 2013Article
- An inverse power-law distribution of molecular bond lifetimes predicts fractional derivative viscoelasticity in biological tissue.Biophysical journal · 2013Article
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Authors and funding
6 authors.
Funding
Abstract
The demembranated (skinned) muscle fiber preparation is widely used to investigate muscle contraction because the intracellular ionic conditions can be precisely controlled. However, plasma membrane removal results in a loss of osmotic regulation, causing abnormal hydration of the myofilament lattice and its proteins. We investigated the structural and functional consequences of varied myofilament lattice spacing and protein hydration on cross-bridge rates of force development and detachment in Drosophila melanogaster indirect flight muscle, using x-ray diffraction to compare the lattice spacing of dissected, osmotically compressed skinned fibers to native muscle fibers in living flies. Osmolytes of different sizes and exclusion properties (Dextran T-500 and T-10) were used to differentially alter lattice spacing and protein hydration. At in vivo lattice spacing, cross-bridge attachment time (t(on)) increased with higher osmotic pressures, consistent with a reduced cross-bridge detachment rate as myofilament protein hydration decreased. In contrast, in the swollen lattice, t(on) decreased with higher osmotic pressures. These divergent responses were reconciled using a structural model that predicts t(on) varies inversely with thick-to-thin filament surface distance, suggesting that cross-bridge rates of force development and detachment are modulated more by myofilament lattice geometry than protein hydration. Generalizing these findings, our results suggest that cross-bridge cycling rates slow as thick-to-thin filament surface distance decreases with sarcomere lengthening, and likewise, cross-bridge cycling rates increase during sarcomere shortening. Together, these structural changes may provide a mechanism for altering cross-bridge performance throughout a contraction-relaxation cycle.
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