ArticleBiophysical journal2012
PIP3 waves and PTEN dynamics in the emergence of cell polarity.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 86 citations in OpenAlex.
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.bioRxiv : the preprint server for biology · 2025Article
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- Signaling and actin waves at a glance.Journal of cell science · 2025Review
- Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration.Nature communications · 2025Article
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- Spatiotemporal distribution of PTEN before directed cell migration in monolayers.In vitro cellular & developmental biology. Animal · 2024Article
- Nonlinear dynamics in phosphoinositide metabolism.Current opinion in cell biology · 2024Review
- Chemotaxis of Large Multinucleate Cells of Dictyostelium Produced by Electric-Pulse Induced Fusion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- A dynamic partitioning mechanism polarizes membrane protein distribution.Nature communications · 2023Article
- Fluctuations of formin binding in the generation of membrane patterns.Biophysical journal · 2023Article
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- Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration.Nature cell biology · 2022Article
- Spontaneous transitions between amoeboid and keratocyte-like modes of migration.Frontiers in cell and developmental biology · 2022Article
- Mechanochemical subcellular-element model of crawling cells.Frontiers in cell and developmental biology · 2022Article
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- Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration inFrontiers in cell and developmental biology · 2021Review
- Local Membrane Curvature Pins and Guides Excitable Membrane Waves in Chemotactic and Macropinocytic Cells - Biomedical Insights From an Innovative Simple Model.Frontiers in cell and developmental biology · 2021Article
- Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a motile eukaryotic cell, front protrusion and tail retraction are superimposed on each other. To single out mechanisms that result in front to tail or in tail to front transition, we separated the two processes in time using cells that oscillate between a full front and a full tail state. State transitions were visualized by total internal reflection fluorescence microscopy using as a front marker PIP3 (phosphatidylinositol [3,4,5] tris-phosphate), and as a tail marker the tumor-suppressor PTEN (phosphatase tensin homolog) that degrades PIP3. Negative fluctuations in the PTEN layer of the membrane gated a local increase in PIP3. In a subset of areas lacking PTEN (PTEN holes), PIP3 was amplified until a propagated wave was initiated. Wave propagation implies that a PIP3 signal is transmitted by a self-sustained process, such that the temporal and spatial profiles of the signal are maintained during passage of the wave across the entire expanse of the cell membrane. Actin clusters were remodeled into a ring along the perimeter of the expanding PIP3 wave. The reverse transition of PIP3 to PTEN was linked to the previous site of wave initiation: where PIP3 decayed first, the entry of PTEN was primed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.