ArticleExperimental and therapeutic medicine2010
Tubulin α-6 chain is a stably expressed reference gene in archival samples of normal oral tissue and oral squamous cell carcinoma.
Article in Experimental and therapeutic medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- LINC00514 regulates oral squamous cell carcinoma progression and prognosis by targeting miR-195-5p.BMC oral health · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Predicting the clinical outcome of oral potentially malignant disorders using transcriptomic-based molecular pathology.British journal of cancer · 2021Article
- Selection of Reliable Reference Genes for RT-qPCR Analysis ofFrontiers in genetics · 2018Article
- Identifying and analyzing different cancer subtypes using RNA-seq data of blood platelets.Oncotarget · 2017Article
- Validation of RPS13 as a reference gene for absolute quantification of SIV RNA in tissue of rhesus macaques.Journal of virological methods · 2016Article
- Expression of CXCL10 is associated with response to radiotherapy and overall survival in squamous cell carcinoma of the tongue.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
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5 authors.
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Abstract
One of the most critical factors in gene expression studies using quantitative real-time PCR is the choice of reference gene. Many of the commonly used reference genes have been shown to vary during a number of biological processes as well as between tissues. It is therefore important to always verify the stability of the gene of choice for all new tissues and experimental conditions. Here, we used two publicly available computer software packages (GeNorm and NormFinder) to investigate the stability of eight potential reference genes in formalin-fixed paraffin-embedded (FFPE) samples from normal oral tissue of different origin as well as from oral squamous cell carcinomas. Both programs found the tubulin α-6 chain (TUBA6) and ribosomal protein S13 (RPS13) to have the most stable expression between malignant and non-malignant tissue. NormFinder also found TUBA6 to be the most stable gene when samples were grouped according to tissue origin. FFPE samples constitute a large research resource, which considerably increases the number of samples available for analysis, leading to more reliable conclusions. Verification of a proper reference gene in oral FFPE tissue is therefore of great importance for future studies.
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