ArticleJournal of clinical microbiology2012
Optimization of matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis for bacterial identification.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Evaluating the Autof MS2600 vs. Biotyper Sirius MALDI-TOF MS: a head-to-head study for routine microbial identification in a university clinical microbiology laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Antimicrobial Resistance inPathogens (Basel, Switzerland) · 2026Article
- Feasibility of anaerobic co-digestion for biogas production from recycled paper industry sludge: optimization of mixing ratios and application in two-stage CSTR system design.Water science and technology : a journal of the International Association on Water Pollution Research · 2025Article
- Evaluation of MALDI-TOF for identification of Vibrio cholerae and Vibrio parahaemolyticus from growth on agar media.Applied microbiology and biotechnology · 2025Article
- Low incidence of cytolysin-positiveGut microbes reports · 2025Article
- Distinguishing methicillin-resistant Staphylococcus aureus from methicillin-sensitive strains by combining FeMikrochimica acta · 2024Article
- Evaluation of the Zybio EXS3000 mass spectrometry in routine identification of Clinical isolates.Heliyon · 2023Article
- Differentiation betweenInternational journal of molecular sciences · 2023Article
- Genotype classification ofFrontiers in microbiology · 2022Article
- Performance of MALDI-TOF Mass Spectrometry in the Philippines.Tropical medicine and infectious disease · 2021Article
- Clinical Laboratory Perspective on Streptococcus halichoeri, an Unusual Nonhemolytic, Lancefield Group B Streptococcus Causing Human Infections.Emerging infectious diseases · 2021Review
- Longitudinal assessment of the bovine ocular bacterial community dynamics in calves.Animal microbiome · 2021Article
- MALDI-TOF MS Biomarker Detection Models to Distinguish RTX Toxin Phenotypes ofFrontiers in cellular and infection microbiology · 2021Article
- Molecular phylogenetic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of isolates from horses identified asJournal of equine science · 2020Article
- Is whole genome sequencing the answer for identifyingBMJ case reports · 2019Article
- Article
- Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in U.S. drinking water.Scientific reports · 2019Article
- Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus.BMC research notes · 2019Article
- Article
- Compatibility of Maximum-Containment Virus-Inactivation Protocols With Identification of Bacterial Coinfections by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.The Journal of infectious diseases · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a relatively new addition to the clinical microbiology laboratory. The performance of the MALDI Biotyper system (Bruker Daltonics) was compared to those of phenotypic and genotypic identification methods for 690 routine and referred clinical isolates representing 102 genera and 225 unique species. We systematically compared direct-smear and extraction methods on a taxonomically diverse collection of isolates. The optimal score thresholds for bacterial identification were determined, and an approach to address multiple divergent results above these thresholds was evaluated. Analysis of identification scores revealed optimal species- and genus-level identification thresholds of 1.9 and 1.7, with 91.9% and 97.0% of isolates correctly identified to species and genus levels, respectively. Not surprisingly, routinely encountered isolates showed higher concordance than did uncommon isolates. The extraction method yielded higher scores than the direct-smear method for 78.3% of isolates. Incorrect species were reported in the top 10 results for 19.4% of isolates, and although there was no obvious cutoff to eliminate all of these ambiguities, a 10% score differential between the top match and additional species may be useful to limit the need for additional testing to reach single-species-level identifications.
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