Evidence map›Paper›PMID 22993176›Full record

ArticleJournal of clinical microbiology2012

Identification of clinical isolates of α-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison.

Angharad Puw Davies, Michelle Reid, Stephen J Hadfield, Stuart Johnston, Jane Mikhail, Llinos G Harris, Howard F Jenkinson, Nidhika Berry, Ann M Lewis, Khalid El-Bouri and 1 more

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Performance of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for rapid identification of streptococci: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017
    Pooled it
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  5. Review
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  7. Antibiotic Susceptibility ofPolish journal of microbiology · 2019
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  14. Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of viridans group streptococci.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Angharad Puw DaviesMedical Microbiology and Infectious Diseases, Institute of Life Science, The College of Medicine, Swansea University, Swansea, United Kingdom. angharad.p.davies@swansea.ac.uk
Michelle Reid
Stephen J Hadfield
Stuart Johnston
Jane Mikhail
Llinos G Harris
Howard F Jenkinson
Nidhika Berry
Ann M Lewis
Khalid El-Bouri
Dietrich Mack

Funding

Medical Research Council G0801929
6 · The paper itself

Abstract

Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.

Indexed as

Bacterial Typing TechniquesDNA, BacterialDNA, IntergenicDNA, RibosomalHumansRNA, Ribosomal, 16SSequence Analysis, DNASpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStreptococcal InfectionsStreptococcusDNA, BacterialDNA, IntergenicDNA, RibosomalRNA, Ribosomal, 16S

Identifiers

PMID22993176
PMCPMC3502998

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.