ArticleJournal of virology2012
Characterization of conserved region 2-deficient mutants of the cytomegalovirus egress protein pM53.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- The nuclear egress complex of Epstein-Barr virus buds membranes through an oligomerization-driven mechanism.PLoS pathogens · 2022Article
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- WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress.Journal of virology · 2018Article
- A Role for Myosin Va in Human Cytomegalovirus Nuclear Egress.Journal of virology · 2018Article
- Review
- Getting to and through the inner nuclear membrane during herpesvirus nuclear egress.Current opinion in cell biology · 2017Review
- Article
- Unexpected features and mechanism of heterodimer formation of a herpesvirus nuclear egress complex.The EMBO journal · 2015Article
- Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Herpes Simplex Virus Protein pUL31 Escorts Nucleocapsids to Sites of Nuclear Egress, a Process Coordinated by Its N-Terminal Domain.PLoS pathogens · 2015Article
- Functional characterization of nuclear trafficking signals in pseudorabies virus pUL31.Journal of virology · 2015Article
- A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Human cytomegalovirus UL50 and UL53 recruit viral protein kinase UL97, not protein kinase C, for disruption of nuclear lamina and nuclear egress in infected cells.Journal of virology · 2014Article
- A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes.PloS one · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dominant-negative (DN) mutants are powerful tools for studying essential protein-protein interactions. A systematic genetic screen of the essential murine cytomegalovirus (MCMV) protein pM53 identified the accumulation of inhibitory mutations within conserved region 2 (CR2) and CR4. The strong inhibitory potential of these CR4 mutants is characterized by a particular phenotype. The DN effect of the small insertion mutations in CR2 was too weak to analyze (M. Popa, Z. Ruzsics, M. Lötzerich, L. Dölken, C. Buser, P. Walther, and U. H. Koszinowski, J. Virol. 84:9035-9046, 2010); therefore, the present study describes the construction of M53 alleles lacking CR2 (either completely or partially) and subsequent examination of the DN effect on MCMV replication upon conditional expression. Overexpression of CR2-deficient pM53 inhibited virus production by about 10,000-fold. This was due to interference with capsid export from the nucleus and viral genome cleavage/packaging. In addition, the fate of the nuclear envelopment complex in the presence of DN pM53 overexpression was analyzed. The CR2 mutants were able to bind to pM50, albeit to a lesser extent than the wild-type protein, and relocalized the wild-type nuclear envelope complex in infected cells. Unlike the CR4 DN, the CR2 DN mutants did not affect the stability of pM50.
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