ArticleJournal of the American Society for Mass Spectrometry2012
MALDI-MS/MS with traveling wave ion mobility for the structural analysis of N-linked glycans.
Article in Journal of the American Society for Mass Spectrometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Sensitive Fluorescence Quantitation and Efficient Free Radical Characterization ofAnalytical chemistry · 2025Article
- Advanced Mass Spectrometry Techniques for the Characterization of Carbohydrates.Handbook of experimental pharmacology · 2025Review
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective.Journal of mass spectrometry : JMS · 2020Article
- MALDI-2 for the Enhanced Analysis ofAnalytical chemistry · 2020Article
- Free-Radical-Mediated Glycan Isomer Differentiation.Analytical chemistry · 2020Article
- Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.Analytica chimica acta · 2020Review
- The expanding role of mass spectrometry in the field of vaccine development.Mass spectrometry reviews · 2020Review
- In-depth structural analysis of glycans in the gas phase.Chemical science · 2019Review
- Structural Studies of Fucosylated N-Glycans by Ion Mobility Mass Spectrometry and Collision-Induced Fragmentation of Negative Ions.Journal of the American Society for Mass Spectrometry · 2018Article
- Article
- Delineation of disease phenotypes associated with esophageal adenocarcinoma by MALDI-IMS-MS analysis of serum N-linked glycans.The Analyst · 2017Article
- Characterization of O-acetylation in sialoglycans by MALDI-MS using a combination of methylamidation and permethylation.Scientific reports · 2017Article
- Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach.Methods in enzymology · 2017Article
- Travelling-wave ion mobility mass spectrometry and negative ion fragmentation of hybrid and complex N-glycans.Journal of mass spectrometry : JMS · 2016Article
- Eradicating mass spectrometric glycan rearrangement by utilizing free radicals.Chemical science · 2016Article
- GlycoMob: an ion mobility-mass spectrometry collision cross section database for glycomics.Glycoconjugate journal · 2016Article
- Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans.Journal of mass spectrometry : JMS · 2016Article
- Characteristic Changes in Cell Surface Glycosylation Accompany Intestinal Epithelial Cell (IEC) Differentiation: High Mannose Structures Dominate the Cell Surface Glycome of Undifferentiated Enterocytes.Molecular & cellular proteomics : MCP · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preference for singly charged ion formation by MALDI makes it a better choice than electrospray ionization for profiling mixtures of N-glycans. For structural analysis, fragmentation of negative ions often yields more informative spectra than fragmentation of positive ones but such ions are more difficult to produce from neutral glycans under MALDI conditions. This work investigates conditions for the formation of both positive and negative ions by MALDI from N-linked glycans released from glycoproteins and their subsequent MS/MS and ion mobility behaviour. 2,4,6-Trihydroxyacetophenone (THAP) doped with ammonium nitrate was found to give optimal ion yields in negative ion mode. Ammonium chloride or phosphate also yielded prominent adducts but anionic carbohydrates such as sulfated N-glycans tended to ionize preferentially. Carbohydrates adducted with all three adducts (phosphate, chloride, and nitrate) produced good negative ion CID spectra but those adducted with iodide and sulfate did not yield fragment ions although they gave stronger signals. Fragmentation paralleled that seen following electrospray ionization providing superior spectra than could be obtained by PSD on MALDI-TOF instruments or with ion traps. In addition, ion mobility drift times of the adducted glycans and the ability of this technique to separate isomers also mirrored those obtained following ESI sample introduction. Ion mobility also allowed profiles to be obtained from samples whose MALDI spectra showed no evidence of such ions allowing the technique to be used in conditions where sample amounts were limiting. The method was applied to N-glycans released from the recombinant human immunodeficiency virus glycoprotein, gp120.
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Registered trials
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