ArticleAmerican journal of physiology. Cell physiology2012
Quantitative phosphoproteomics in nuclei of vasopressin-sensitive renal collecting duct cells.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Bayesian mapping of protein kinases to vasopressin-regulated phosphorylation sites in renal collecting duct.American journal of physiology. Renal physiology · 2024Article
- Proteomic analysis of murine kidney proximal tubule sub-segment derived cell lines reveals preferences in mitochondrial pathway activity.Journal of proteomics · 2023Article
- Phosphoproteomic Identification of Vasopressin/cAMP/Protein Kinase A-Dependent Signaling in Kidney.Molecular pharmacology · 2021Review
- Phosphoproteomic identification of vasopressin-regulated protein kinases in collecting duct cells.British journal of pharmacology · 2021Article
- CRISPR-Cas9/phosphoproteomics identifies multiple noncanonical targets of myosin light chain kinase.American journal of physiology. Renal physiology · 2020Article
- From Molecules to Mechanisms: Functional Proteomics and Its Application to Renal Tubule Physiology.Physiological reviews · 2018Review
- Proteomic determination of the lysine acetylome and phosphoproteome in the rat native inner medullary collecting duct.Physiological genomics · 2018Article
- Genome-Wide Mapping of DNA Accessibility and Binding Sites for CREB and C/EBPJournal of the American Society of Nephrology : JASN · 2018Article
- Identification of β-catenin-interacting proteins in nuclear fractions of native rat collecting duct cells.American journal of physiology. Renal physiology · 2017Article
- Proteomic and phosphoproteomic analysis of renal cortex in a salt-load rat model of advanced kidney damage.Scientific reports · 2016Article
- Systems-level analysis reveals selective regulation of Aqp2 gene expression by vasopressin.Scientific reports · 2016Article
- Proteomic profiling of nuclear fractions from native renal inner medullary collecting duct cells.Physiological genomics · 2016Article
- Deep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling.American journal of physiology. Cell physiology · 2015Article
- Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions.American journal of physiology. Cell physiology · 2015Article
- Use of LC-MS/MS and Bayes' theorem to identify protein kinases that phosphorylate aquaporin-2 at Ser256.American journal of physiology. Cell physiology · 2014Article
- Vasopressin-2 receptor signaling and autosomal dominant polycystic kidney disease: from bench to bedside and back again.Journal of the American Society of Nephrology : JASN · 2014Review
- Proteomic pearl diving versus systems biology in cell physiology. Focus on "proteomic mapping of proteins released during necrosis and apoptosis from cultured neonatal cardiac myocytes".American journal of physiology. Cell physiology · 2014Article
- Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct.American journal of physiology. Renal physiology · 2014Article
- Vasopressin and the regulation of aquaporin-2.Clinical and experimental nephrology · 2013Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Vasopressin regulates transport across the collecting duct epithelium in part via effects on gene transcription. Transcriptional regulation occurs partially via changes in phosphorylation of transcription factors, transcriptional coactivators, and protein kinases in the nucleus. To test whether vasopressin alters the nuclear phosphoproteome of vasopressin-sensitive cultured mouse mpkCCD cells, we used stable isotope labeling and mass spectrometry to quantify thousands of phosphorylation sites in nuclear extracts and nuclear pellet fractions. Measurements were made in the presence and absence of the vasopressin analog dDAVP. Of the 1,251 sites quantified, 39 changed significantly in response to dDAVP. Network analysis of the regulated proteins revealed two major clusters ("cell-cell adhesion" and "transcriptional regulation") that were connected to known elements of the vasopressin signaling pathway. The hub proteins for these two clusters were the transcriptional coactivator β-catenin and the transcription factor c-Jun. Phosphorylation of β-catenin at Ser552 was increased by dDAVP [log(2)(dDAVP/vehicle) = 1.79], and phosphorylation of c-Jun at Ser73 was decreased [log(2)(dDAVP/vehicle) = -0.53]. The β-catenin site is known to be targeted by either protein kinase A or Akt, both of which are activated in response to vasopressin. The c-Jun site is a canonical target for the MAP kinase Jnk2, which is downregulated in response to vasopressin in the collecting duct. The data support the idea that vasopressin-mediated control of transcription in collecting duct cells involves selective changes in the nuclear phosphoproteome. All data are available to users at http://helixweb.nih.gov/ESBL/Database/mNPPD/.
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