ArticlePlant physiology2012
Proteasome-mediated turnover of Arabidopsis MED25 is coupled to the activation of FLOWERING LOCUS T transcription.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- RDO3 REPRESSOR 27, a new MED25 allele, regulates seed dormancy dependent on DOG1 and ABA pathways in Arabidopsis.Planta · 2026Article
- When MED16 Meets Plant Growth, Development, and Stress Response.International journal of molecular sciences · 2026Review
- Integrative transcriptome and genome resequencing reveals conserved flowering regulators and allelic variants in early- and late-flowering linseed (Linum usitatissimum L.) accessions.Scientific reports · 2026Article
- An Australian chickpea pan-genome provides insights into genome organization and offers opportunities for enhancing drought adaptation for crop improvement.Plant biotechnology journal · 2025Article
- A multiprotein regulatory module, MED16-MBR1&2, controls MED25 homeostasis during jasmonate signaling.Nature communications · 2025Article
- Geography, altitude, agriculture, and hypoxia.Plant physiology · 2024Review
- Environmental genome-wide association studies across precipitation regimes reveal that the E3 ubiquitin ligase MBR1 regulates plant adaptation to rainy environments.Plant communications · 2024Article
- Article
- Regulation of Flowering Time by Environmental Factors in Plants.Plants (Basel, Switzerland) · 2023Review
- The Mediator complex subunit MED25 interacts with HDA9 and PIF4 to regulate thermomorphogenesis.Plant physiology · 2023Article
- Compressed variance component mixed model reveals epistasis associated with flowering inFrontiers in plant science · 2023Article
- ZmTE1 promotes plant height by regulating intercalary meristem formation and internode cell elongation in maize.Plant biotechnology journal · 2022Article
- Genetic control and allele variation among soybean maturity groups 000 through IX.The plant genome · 2021Article
- The Important Function of Mediator Complex in Controlling the Developmental Transitions in Plants.International journal of molecular sciences · 2020Review
- Silencing SlMED18, tomato Mediator subunit 18 gene, restricts internode elongation and leaf expansion.Scientific reports · 2018Article
- The Arabidopsis RING-Type E3 Ligase TEAR1 Controls Leaf Development by Targeting the TIE1 Transcriptional Repressor for Degradation.The Plant cell · 2017Article
- The Multitalented MEDIATOR25.Frontiers in plant science · 2017Review
- Bottom-up Assembly of the Phytochrome Network.PLoS genetics · 2016Article
- Integration of Light and Photoperiodic Signaling in Transcriptional Nuclear Foci.Developmental cell · 2015Article
- HEMERA Couples the Proteolysis and Transcriptional Activity of PHYTOCHROME INTERACTING FACTORs in Arabidopsis Photomorphogenesis.The Plant cell · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Mediator complex is a greater than 1-megadalton complex, composed of about 30 subunits and found in most eukaryotes, whose main role is to transmit signals from DNA-bound transcription factors to RNA Polymerase II. The proteasome is emerging as an important regulator of transcription during both initiation and elongation. It is increasing the number of cases where the proteolysis of transcriptional activators by the proteasome activates their function. This counterintuitive phenomenon was called "activation by destruction." Here, we show that, in Arabidopsis (Arabidopsis thaliana), PHYTOCHROME AND FLOWERING TIME1 (PFT1), the MEDIATOR25 (MED25) subunit of the plant Mediator complex, is degraded by the proteasome and that proteasome-mediated PFT1 turnover is coupled to its role in stimulating the transcription of FLOWERING LOCUS T, the plant florigen, which is involved in the process of flowering induction. We further identify two novel RING-H2 proteins that target PFT1 for degradation. We show that MED25-BINDING RING-H2 PROTEIN1 (MBR1) and MBR2 bind to PFT1 in yeast (Saccharomyces cerevisiae) and in vitro, and they promote PFT1 degradation in vivo, in a RING-H2-dependent way, typical of E3 ubiquitin ligases. We further show that both MBR1 and MBR2 also promote flowering by PFT1-dependent and -independent mechanisms. Our findings extend the phenomenon of activation by destruction to a Mediator subunit, adding a new mechanism by which Mediator subunits may regulate downstream genes in specific pathways. Furthermore, we show that two novel RING-H2 proteins are involved in the destruction of PFT1, adding new players to this process in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.