ArticleJournal of cell science2012
Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Myosin-I's motor and actin assembly activation activities are modular and separable in budding yeast clathrin-mediated endocytosis.microPublication biology · 2024Article
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- Nanotechnology Approaches for Enhanced CNS Drug Delivery in the Management of Schizophrenia.Advanced pharmaceutical bulletin · 2022Review
- Regulation of the Total Cell Surface Area in DividingFrontiers in cell and developmental biology · 2020Article
- Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL inMolecular biology of the cell · 2019Article
- High resolution measurement of membrane receptor endocytosis.Journal of biological methods · 2018Article
- Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model.Nanomaterials (Basel, Switzerland) · 2017Article
- The long life of an endocytic patch that misses AP-2.Current genetics · 2016Review
- Evolutionary Changes on the Way to Clathrin-Mediated Endocytosis in Animals.Genome biology and evolution · 2016Article
- ENTH and ANTH domain proteins participate in AP2-independent clathrin-mediated endocytosis.Journal of cell science · 2015Article
- Imaging and modeling the dynamics of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Rab11 regulates trafficking of trans-sialidase to the plasma membrane through the contractile vacuole complex of Trypanosoma cruzi.PLoS pathogens · 2014Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Resolving the homology-function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology.Molecular and biochemical parasitologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The protein clathrin mediates one of the major pathways of endocytosis from the extracellular milieu and plasma membrane. In single-cell eukaryotes, such as Saccharomyces cerevisiae, the gene encoding clathrin is not an essential gene, raising the question of whether clathrin conveys specific advantages for multicellularity. Furthermore, in contrast to mammalian cells, endocytosis in S. cerevisiae is not dependent on either clathrin or adaptor protein 2 (AP2), an endocytic adaptor molecule. In this study, we investigated the requirement for components of clathrin-mediated endocytosis (CME) in another unicellular organism, the amoeba Dictyostelium. We identified a heterotetrameric AP2 complex in Dictyostelium that is similar to that which is found in higher eukaryotes. By simultaneously imaging fluorescently tagged clathrin and AP2, we found that, similar to higher eukaryotes, these proteins colocalized to membrane puncta that move into the cell together. In addition, the contractile vacuole marker protein, dajumin-green fluorescent protein (GFP), is trafficked via the cell membrane and internalized by CME in a clathrin-dependent, AP2-independent mechanism. This pathway is distinct from other endocytic mechanisms in Dictyostelium. Our finding that CME is required for the internalization of contractile vacuole proteins from the cell membrane explains the contractile vacuole biogenesis defect in Dictyostelium cells lacking clathrin. Our results also suggest that the machinery for CME and its role in organelle maintenance appeared early during eukaryotic evolution. We hypothesize that dependence of endocytosis on specific components of the CME pathway evolved later, as demonstrated by internalization independent of AP2 function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.