ArticleTissue engineering. Part C, Methods2013
Tissue-engineered constructs of human oral mucosa examined by Raman spectroscopy.
Article in Tissue engineering. Part C, Methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Raman spectroscopic modality to examine therapeutic efficacy of Galectin-3 inhibitor in prostate cancer.Biochemical and biophysical research communications · 2025Article
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- Oral mucosa equivalents, prevascularization approaches, and potential applications.Connective tissue research · 2022Review
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
- Telomere Length Shortening in Microglia: Implication for Accelerated Senescence and Neurocognitive Deficits in HIV.Vaccines · 2021Article
- Iron-binding cellular profile of transferrin using label-free Raman hyperspectral imaging and singular value decomposition (SVD).Free radical biology & medicine · 2021Article
- Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct SuccessTissue engineering. Part C, Methods · 2021Article
- Quantitative label-free imaging of iron-bound transferrin in breast cancer cells and tumors.Redox biology · 2020Article
- Optical Metric Assessed Engineered Tissues Over a Range of Viability States.Tissue engineering. Part C, Methods · 2019Article
- Methamphetamine-induced apoptosis in glial cells examined under marker-free imaging modalities.Journal of biomedical optics · 2019Article
- Noninvasive Optical Assessment of Implanted Engineered Tissues Correlates with Cytokine Secretion.Tissue engineering. Part C, Methods · 2018Article
- Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy.Biomaterials · 2017Article
- Human oral mucosa tissue-engineered constructs monitored by Raman fiber-optic probe.Tissue engineering. Part C, Methods · 2015Article
- High-frequency ultrasonic imaging of growth and development in manufactured engineered oral mucosal tissue surfaces.Ultrasound in medicine & biology · 2014Article
- Soft tissue engineering in craniomaxillofacial surgery.Annals of maxillofacial surgery · 2014Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
A noninvasive quality monitoring of tissue-engineered constructs is a required component of any successful tissue-engineering technique. During a 2-week production period, ex vivo produced oral mucosa-equivalent constructs (EVPOMEs) may encounter adverse culturing conditions that might compromise their quality and render them ineffective. We demonstrate the application of near-infrared Raman spectroscopy to in vitro monitoring of EVPOMEs during their manufacturing process, with the ultimate goal of applying this technology in situ to monitor the grafted EVPOMEs. We identify Raman spectroscopic failure indicators for less-than optimal EVPOMEs that are stressed by higher temperature and exposure to higher than normal concentration of calcium ions. Raman spectra of EVPOMEs exposed to thermal and calcium stress showed correlation of the band height ratio of CH(2) deformation to phenylalanine ring breathing modes, providing a Raman metric to distinguish between viable and nonviable constructs. We compared these results to histology and glucose consumption measurements, demonstrating that Raman spectroscopy is more sensitive and specific to changes in proteins' secondary structure not visible by H&E histology. We also exposed the EVPOMEs to rapamycin, a cell growth inhibitor and cell proliferation capacity preserver, and distinguished between EVPOMEs pretreated with 2 nM rapamycin and controls, using the ratio of the Amide III envelope to the phenylalanine band as an indicator.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.