ArticleTissue engineering. Part A2013
Design and validation of a bioreactor for simulating the cardiac niche: a system incorporating cyclic stretch, electrical stimulation, and constant perfusion.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Novel, low-cost bioreactor forFrontiers in bioengineering and biotechnology · 2025Article
- Bridging the Gap: Advances and Challenges in Heart Regeneration from In Vitro to In Vivo Applications.Bioengineering (Basel, Switzerland) · 2024Review
- Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch.Medical science monitor : international medical journal of experimental and clinical research · 2021Review
- Insights to Heart Development and Cardiac Disease Models Using Pluripotent Stem Cell Derived 3D Organoids.Frontiers in cell and developmental biology · 2021Review
- BEaTS-α an open access 3D printed device for in vitro electromechanical stimulation of human induced pluripotent stem cells.Scientific reports · 2020Article
- Enhancing all-in-one bioreactors by combining interstitial perfusion, electrical stimulation, on-line monitoring and testing within a single chamber for cardiac constructs.Scientific reports · 2018Article
- Unravelling the effects of mechanical physiological conditioning on cardiac adipose tissue-derived progenitor cells in vitro and in silico.Scientific reports · 2018Article
- Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction.Stem cells translational medicine · 2017Article
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
- "The state of the heart": Recent advances in engineering human cardiac tissue from pluripotent stem cells.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Establishing Early Functional Perfusion and Structure in Tissue Engineered Cardiac Constructs.Critical reviews in biomedical engineering · 2015Review
- Induced pluripotent stem cells for regenerative medicine.Annual review of biomedical engineering · 2014Review
- Mimicking isovolumic contraction with combined electromechanical stimulation improves the development of engineered cardiac constructs.Tissue engineering. Part A · 2014Article
- Origin, development, and differentiation of cardiac fibroblasts.Journal of molecular and cellular cardiology · 2014Review
- It's all in the timing: modeling isovolumic contraction through development and disease with a dynamic dual electromechanical bioreactor system.Organogenesis · 2014Article
- Cardiac cell culture model as a left ventricle mimic for cardiac tissue generation.Analytical chemistry · 2013Article
- Biophysical cues enhance myogenesis of human adipose derived stem/stromal cells.Biochemical and biophysical research communications · 2013Article
- Review
- The use of a novel cardiac bioreactor system in investigating fibroblast physiology and its perspectives.OrganogenesisArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To simulate the cardiac niche, a bioreactor system was designed and constructed to incorporate cyclic stretch, rhythmic electrical stimulation, and constant perfusion. The homogeneity of surface strain distribution across the cell culture substrate was confirmed with ARAMIS deformation analysis. The proliferation marker, Ki-67, detected in human umbilical vein endothelial cells and 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide cytotoxicity assay performed on human atrial fibroblasts confirmed biocompatibility of this novel device. Cyclic stretch treatment for 24 h resulted in the perpendicular alignment of human atrial fibroblasts. An electrical stimulation system containing carbon electrodes was characterized by electrochemical impedance spectroscopy and charge injection/recovery studies, which indicated that increased corrosive reactions were associated with a higher input voltage and prolonged pulse duration. Field stimulation delivered through this system could induce rhythmic contractions in adult rat ventricular myocytes, with contractile characteristics similar to those paced in a standard field stimulation chamber. In conclusion, this bioreactor provides a novel tool to study the interaction between physical stimulation and cardiac cell physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.