ArticleJournal of lipid research2012
C2 domain membrane penetration by group IVA cytosolic phospholipase A₂ induces membrane curvature changes.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Decoding protein-phospholipid interaction networks in cancer: the role of acyl-chain remodeling.RSC chemical biology · 2026Review
- cPLAScience advances · 2026Article
- Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration.Nature communications · 2023Article
- Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells.Blood advances · 2023Article
- Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation.Nature cell biology · 2022Article
- The Cytosolic Phospholipase ABiomolecules · 2020Article
- ER membranes exhibit phase behavior at sites of organelle contact.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase AeLife · 2019Article
- Modeling Membrane Curvature Generation due to Membrane⁻Protein Interactions.Biomolecules · 2018Review
- Role of Munc13-4 as a Ca2+-dependent tether during platelet secretion.The Biochemical journal · 2016Article
- Structural and Functional Analysis of Calcium Ion Mediated Binding of 5-Lipoxygenase to Nanodiscs.PloS one · 2016Article
- The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes.The Journal of biological chemistry · 2014Article
- Synaptotagmin's role in neurotransmitter release likely involves Ca(2+)-induced conformational transition.Biophysical journal · 2014Article
- Cellular and molecular interactions of phosphoinositides and peripheral proteins.Chemistry and physics of lipids · 2014Review
- The Ebola virus matrix protein deeply penetrates the plasma membrane: an important step in viral egress.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Group IVA cytosolic phospholipase A(2) (cPLA(2)α) is an 85 kDa enzyme that regulates the release of arachidonic acid (AA) from the sn-2 position of membrane phospholipids. It is well established that cPLA(2)α binds zwitterionic lipids such as phosphatidylcholine in a Ca(2+)-dependent manner through its N-terminal C2 domain, which regulates its translocation to cellular membranes. In addition to its role in AA synthesis, it has been shown that cPLA(2)α promotes tubulation and vesiculation of the Golgi and regulates trafficking of endosomes. Additionally, the isolated C2 domain of cPLA(2)α is able to reconstitute Fc receptor-mediated phagocytosis, suggesting that C2 domain membrane binding is sufficient for phagosome formation. These reported activities of cPLA(2)α and its C2 domain require changes in membrane structure, but the ability of the C2 domain to promote changes in membrane shape has not been reported. Here we demonstrate that the C2 domain of cPLA(2)α is able to induce membrane curvature changes to lipid vesicles, giant unilamellar vesicles, and membrane sheets. Biophysical assays combined with mutagenesis of C2 domain residues involved in membrane penetration demonstrate that membrane insertion by the C2 domain is required for membrane deformation, suggesting that C2 domain-induced membrane structural changes may be an important step in signaling pathways mediated by cPLA(2)α.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.