ArticleNature communications2012
Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
120 citing papers in PubMed, 1 synthesis or guideline pooled it, 188 citations in OpenAlex.
- Pooled it
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- Reversible epiblast regionalization determines differentiation potential of human pluripotent stem cells.Nature biotechnology · 2026Article
- Development of a high-efficiency induction system for embryonic oral epithelium from human pluripotent stem cells.Stem cell reports · 2026Article
- Exploring the impact of human pluripotent stem cell heterogeneity on corneal limbal stem cell differentiation outcomes.Scientific reports · 2026Article
- Human Organoids Reveal PTEN-Driven Mesendoderm Specification via Retinoic Acid Signaling Suppression.Research (Washington, D.C.) · 2026Article
- Article
- Self-organizing models of human trunk organogenesis recapitulate spinal cord and spine co-morphogenesis.Nature biotechnology · 2024Article
- PDX1+ cell budding morphogenesis in a stem cell-derived islet spheroid system.Nature communications · 2024Article
- Comparative study of enriched dopaminergic neurons from siblings with Gaucher disease discordant for parkinsonism.bioRxiv : the preprint server for biology · 2024Article
- Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells.Frontiers in cell and developmental biology · 2024Article
- Article
- Monolayer platform to generate and purify primordial germ-like cells in vitro provides insights into human germline specification.Nature communications · 2023Article
- Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture.Development (Cambridge, England) · 2023Article
- The Foundational Data Initiative for Parkinson Disease: Enabling efficient translation from genetic maps to mechanism.Cell genomics · 2023Article
- Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Virtual cells in a virtual microenvironment recapitulate early development-like patterns in human pluripotent stem cell colonies.Stem cell reports · 2023Article
- Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.Stem cell reports · 2022Article
- Generation of human A9 dopaminergic pacemakers from induced pluripotent stem cells.Molecular psychiatry · 2022Article
- Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids.Development (Cambridge, England) · 2022Article
60 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The pluripotent nature of human embryonic stem cells (hESCs) makes them convenient for deriving therapeutically relevant cells. Here we show using Wnt reporter hESC lines that the cells are heterogeneous with respect to endogenous Wnt signalling activity. Moreover, the level of Wnt signalling activity in individual cells correlates with differences in clonogenic potential and lineage-specific differentiation propensity. The addition of Wnt protein or, conversely, a small-molecule Wnt inhibitor (IWP2) reduces heterogeneity, allowing stable expansion of Wnt(high) or Wnt(low) hESC populations, respectively. On differentiation, the Wnt(high) hESCs predominantly form endodermal and cardiac cells, whereas the Wnt(low) hESCs generate primarily neuroectodermal cells. Thus, heterogeneity with respect to endogenous Wnt signalling underlies much of the inefficiency in directing hESCs towards specific cell types. The relatively uniform differentiation potential of the Wnt(high) and Wnt(low) hESCs leads to faster and more efficient derivation of targeted cell types from these populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.