ArticleThe Journal of clinical investigation1990
Rat aortic smooth muscle cells in culture express kallikrein, kininogen, and bradykininase activity.
Article in The Journal of clinical investigation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 102 citations in OpenAlex.
- Bradykinin, insulin, and glycemia responses to exercise performed above and below lactate threshold in individuals with type 2 diabetes.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Trial
- Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation.JID innovations : skin science from molecules to population health · 2021Article
- Role of Kinins in Hypertension and Heart Failure.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Article
- Bradykinin antagonist counteracts the acute effect of both angiotensin-converting enzyme inhibition and of angiotensin receptor blockade on the lower limit of autoregulation of cerebral blood flow.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2014Article
- Reduced L-carnitine transport in aortic endothelial cells from spontaneously hypertensive rats.PloS one · 2014Article
- The kallikrein-kinin system as a regulator of cardiovascular and renal function.Comprehensive Physiology · 2011Review
- Evidence for prostacyclin and cAMP upregulation by bradykinin and insulin-like growth factor 1 in vascular smooth muscle cells.Journal of receptor and signal transduction research · 2010Article
- Arterial and renal consequences of partial genetic deficiency in tissue kallikrein activity in humans.The Journal of clinical investigation · 2005Article
- Exercise-induced increase in interstitial bradykinin and adenosine concentrations in skeletal muscle and peritendinous tissue in humans.The Journal of physiology · 2002Article
- AT(2) receptor-dependent vasodilation is mediated by activation of vascular kinin generation under flow conditions.British journal of pharmacology · 2002Article
- Endothelial AT(2)-receptors: chicken or egg?British journal of pharmacology · 2002Article
- The mechanism of bradykinin-induced endothelium-dependent contraction and relaxation in the porcine interlobar renal artery.British journal of pharmacology · 2000Article
- Angiotensin II type 2 receptor overexpression activates the vascular kinin system and causes vasodilation.The Journal of clinical investigation · 1999Article
- Tissue kallikreins structure, regulation, and participation in mammalian physiology and disease.Clinical reviews in allergy & immunology · 1998Review
- ACE inhibition, endothelial function and coronary artery lesions. Role of kinins and nitric oxide.Drugs · 1997Review
- Endothelial function and bradykinin in humans.Drugs · 1997Review
- The myostimulating effect of tissue kallikrein on rat uterus.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Pharmacological characterization of rabbit corpus cavernosum relaxation mediated by the tissue kallikrein-kinin system.British journal of pharmacology · 1994Article
- Diverse factors influencing angiotensin metabolism during ACE inhibition: insights from molecular biology and genetic studies.British heart journal · 1994Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
We have studied rat vascular smooth muscle (VSM) cells in culture for the presence of key elements of the glandular kallikrein-kinin system. Direct radioimmunoassay (RIA) using antiserum against rat urinary kallikrein detected a glandular kallikrein-like enzyme (GKLE) in VSM cells and in media. VSM homogenates and culture media had kininogenase activity, generating kinins from dog kininogen. About half of the GKLE was enzymatically inactive which could be activated with trypsin. Kininogenase activity was inhibited completely by aprotinin but only 20% by soybean trypsin inhibitor (SBTI). Trypsin liberated kinins from homogenates and media, demonstrating that VSM cells contain kininogen. Homogenates and media rapidly degrade bradykinin. GKLE, kininogen, and bradykininase activity were all present in VSM cells grown in defined media that contain no serum, thus eliminating any contamination or artefacts from fetal calf serum in standard culture media. Blood vessels of the rat have been reported to contain GKLE. Our observations indicate that GKLE is synthesized by VSM cells, not deposited from plasma. Furthermore, VSM cells synthesize kininogen and bradykininase(s), the other key elements of the glandular kallikrein-kinin system. Thus it is possible that the system functions as an autocoid mechanism that regulates local vascular tone.
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Registered trials
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