ArticleGenesis (New York, N.Y. : 2000)2012
Generation of Dhx9-deficient clones in T-cell development with a mitotic recombination technique.
Article in Genesis (New York, N.Y. : 2000), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- DExD/H-box helicase 9 intrinsically controls CD8Science advances · 2022Article
- A mosaic analysis system with Cre or Tomato expression in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The RNA helicase A in malignant transformation.Oncotarget · 2016Review
- Tracking proliferative history in lymphocyte development with cre-mediated sister chromatid recombination.PLoS genetics · 2013Article
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Authors and funding
6 authors.
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Abstract
Mitotic recombination is an effective tool for generating mutant clones in somatic tissues. Because of difficulties associated with detecting and quantifying mutant clones in mice, this technique is limited to analysis of growth-related phenotypes induced by loss function of tumor suppressor genes. Here, we used the polymorphic CD45.1/CD45.2 alleles on chromosome 1 as pan-hematopoietic markers to track mosaic clones generated through mitotic recombination in developing T cells. We show that lineage-specific mitotic recombination can be induced and reliably detected as CD45.1 or CD45.2 homozygous clones from the CD45.1/CD45.2 heterozygous background. We have applied this system in the analysis of a lethal mutation in the Dhx9 gene. Mosaic analysis revealed a stage-specific role for Dhx9 during T-cell maturation. Thus, the experimental system described in this study offers a practical means for mosaic analysis of germline mutations in the hematopoietic system.
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