ArticleFrontiers in neuroscience2012
Activity of protease-activated receptors in primary cultured human myenteric neurons.
Article in Frontiers in neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Proteases in intestinal health and disease.Nature reviews. Gastroenterology & hepatology · 2026Review
- The enteric nervous system.Physiological reviews · 2023Review
- The gut brain in a dish: Murine primary enteric nervous system cell cultures.Neurogastroenterology and motility · 2022Review
- Inhibition of Serine Proteases as a Novel Therapeutic Strategy for Abdominal Pain in IBS.Frontiers in physiology · 2022Review
- Article
- Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon.Parasitology research · 2018Article
- Article
- Review
- Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche.Stem cells international · 2016Article
- Mechanical stress activates neurites and somata of myenteric neurons.Frontiers in cellular neuroscience · 2015Article
- Role of enteric neurotransmission in host defense and protection of the gastrointestinal tract.Autonomic neuroscience : basic & clinical · 2014Review
- Protease-activated receptors as therapeutic targets in visceral pain.Current neuropharmacology · 2013Article
- Mouse enteric neuronal cell culture.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activity of the four known protease-activated receptors (PARs) has been well studied in rodent enteric nervous system and results in animal models established an important role for neuronal PAR2. We recently demonstrated that, unlike in rodents, PAR1 is the dominant neuronal protease receptor in the human submucous plexus. With this study we investigated whether this also applies to the human myenteric plexus. We used voltage sensitive dye recordings to detect action potential discharge in primary cultures of human myenteric neurons in response to PAR activating peptides (APs). Application of the PAR1-AP (TFLLR) or PAR4-AP (GYPGQV) evoked spike discharge in 79 or 23% of myenteric neurons, respectively. The PAR1-AP response was mimicked by the endogenous PAR1 activator thrombin and blocked by the PAR1 antagonists SCH79797. Human myenteric neurons did not respond to PAR2-AP. This was not due to culture conditions because all three PAR-APs evoked action potentials in cultured guinea pig myenteric neurons. Consecutive application of PAR-APs revealed coexpression (relative to the population responding to PAR-APs) of PAR1/PAR2 in 51%, PAR1/PAR4 in 43%, and of PAR2/PAR4 in 29% of guinea pig myenteric neurons. Our study provided further evidence for the prominent role of neuronal PAR1 in the human enteric nervous system.
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