ArticleThe Journal of physiology2012
Facilitation of cytosolic calcium wave propagation by local calcium uptake into the sarcoplasmic reticulum in cardiac myocytes.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Ceftriaxone alleviates mitochondrial damage through the inhibition of extrasynaptic NMDA receptor-mediated changes in intracellular calcium levels to improve cognitive deficits in APP/PS1 mice.Alzheimer's research & therapy · 2025Article
- The 'Reverse FDUF' Mechanism of Atrial Excitation-Contraction Coupling Sustains Calcium Alternans-A Hypothesis.Biomolecules · 2022Article
- PKC Inhibits Sec61 Translocon-Mediated Sarcoplasmic Reticulum CaFrontiers in physiology · 2022Article
- Increasing SERCA function promotes initiation of calcium sparks and breakup of calcium waves.The Journal of physiology · 2021Article
- CaMKII inhibition reduces arrhythmogenic Ca2+ events in subendocardial cryoinjured rat living myocardial slices.The Journal of general physiology · 2021Article
- Excitation-contraction coupling and calcium release in atrial muscle.Pflugers Archiv : European journal of physiology · 2021Review
- Effect of carvedilol on atrial excitation-contraction coupling, CaAmerican journal of physiology. Heart and circulatory physiology · 2020Article
- Pharmacological Modulation of Mitochondrial CaFrontiers in physiology · 2018Article
- Ambiguous interactions between diastolic and SR CaThe Journal of general physiology · 2017Review
- Perspectives on "Control of Ca release from within the cardiac sarcoplasmic reticulum".The Journal of general physiology · 2017Article
- The intricacies of atrial calcium cycling during excitation-contraction coupling.The Journal of general physiology · 2017Review
- A novel mechanism of tandem activation of ryanodine receptors by cytosolic and SR luminal CaThe Journal of physiology · 2017Article
- In vivo CaPhysiological reports · 2017Article
- Phospholamban ablation rescues the enhanced propensity to arrhythmias of mice with CaMKII-constitutive phosphorylation of RyR2 at site S2814.The Journal of physiology · 2016Article
- Electrically Induced Calcium Handling in Cardiac Progenitor Cells.Stem cells international · 2016Article
- Inositol-1,4,5-trisphosphate induced Ca2+ release and excitation-contraction coupling in atrial myocytes from normal and failing hearts.The Journal of physiology · 2015Article
- Article
- Dantrolene suppresses spontaneous Ca2+ release without altering excitation-contraction coupling in cardiomyocytes of aged mice.American journal of physiology. Heart and circulatory physiology · 2014Article
- SERCA2a gene therapy in heart failure: an anti-arrhythmic positive inotrope.British journal of pharmacology · 2014Review
- Calcium and IP3 dynamics in cardiac myocytes: experimental and computational perspectives and approaches.Frontiers in pharmacology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The widely accepted paradigm for cytosolic Ca(2+) wave propagation postulates a 'fire-diffuse-fire' mechanism where local Ca(2+)-induced Ca(2+) release (CICR) from the sarcoplasmic reticulum (SR) via ryanodine receptor (RyR) Ca(2+) release channels diffuses towards and activates neighbouring release sites, resulting in a propagating Ca(2+) wave. A recent challenge to this paradigm proposed the requirement for an intra-SR 'sensitization' Ca(2+) wave that precedes the cytosolic Ca(2+) wave and primes RyRs from the luminal side to CICR. Here, we tested this hypothesis experimentally with direct simultaneous measurements of cytosolic ([Ca(2+)](i); rhod-2) and intra-SR ([Ca(2+)](SR); fluo-5N) calcium signals during wave propagation in rabbit ventricular myocytes, using high resolution fluorescence confocal imaging. The increase in [Ca(2+)](i) at the wave front preceded depletion of the SR at each point along the calcium wave front, while during this latency period a transient increase of [Ca(2+)](SR) was observed. This transient elevation of [Ca(2+)](SR) could be identified at individual release junctions and depended on the activity of the sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA). Increased SERCA activity (β-adrenergic stimulation with 1 μM isoproterenol (isoprenaline)) decreased the latency period and increased the amplitude of the transient elevation of [Ca(2+)](SR), whereas inhibition of SERCA (3 μM cyclopiazonic acid) had the opposite effect. In conclusion, the data provide experimental evidence that local Ca(2+) uptake by SERCA into the SR facilitates the propagation of cytosolic Ca(2+) waves via luminal sensitization of the RyR, and supports a novel paradigm of a 'fire-diffuse-uptake-fire' mechanism for Ca(2+) wave propagation in cardiac myocytes.
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Registered trials
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