ArticleProceedings of the National Academy of Sciences of the United States of America2012
High-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
119 citing papers in PubMed.
- Ca2+ signals generated by CatSper and Ca2+ stores regulate different behaviors in human sperm.The Journal of biological chemistry · 2013Trial
- Regulation of spermatozoal capacitation by serotonin.The Journal of reproduction and development · 2026Review
- Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.Small methods · 2026Article
- Optimal Chirality Enhances Long-Range Fluctuation-Induced Interactions in Active Fluids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Swimming by Spinning: Spinning-Top Type Rotations Regularize Sperm Swimming Into Persistently Progressive Paths in 3D.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges.Animals : an open access journal from MDPI · 2025Review
- Quantitative phase imaging verification in large field-of-view lensless holographic microscopy via two-photon 3D printing.Scientific reports · 2024Article
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography.Light, science & applications · 2024Article
- Digital in-line holographic microscopy for label-free identification and tracking of biological cells.Military Medical Research · 2024Review
- Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos†.Biology of reproduction · 2024Review
- Progesterone and estradiol regulate sperm hyperactivation and in vitro fertilization success in mice.The Journal of reproduction and development · 2024Article
- All-optical dual module platform for motility-based functional scrutiny of microencapsulated probiotic bacteria.Biomedical optics express · 2024Article
- Quantitative, high-sensitivity measurement of liquid analytes using a smartphone compass.Nature communications · 2024Article
- Roadmap on computational methods in optical imaging and holography [invited].Applied physics. B, Lasers and optics · 2024Review
- Evaluation of women's aging influence on sperm passage inside the fallopian tube using 3D dynamic mechanical modeling.Frontiers in bioengineering and biotechnology · 2024Article
- Artificial intelligence-enabled quantitative phase imaging methods for life sciences.Nature methods · 2023Review
- Microfluidics on lensless, semiconductor optical image sensors: challenges and opportunities for democratization of biosensing at the micro-and nano-scale.Nanophotonics (Berlin, Germany) · 2023Review
- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence.Light, science & applications · 2023Article
- Article
59 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
Dynamic tracking of human sperms across a large volume is a challenging task. To provide a high-throughput solution to this important need, here we describe a lensfree on-chip imaging technique that can track the three-dimensional (3D) trajectories of > 1,500 individual human sperms within an observation volume of approximately 8-17 mm(3). This computational imaging platform relies on holographic lensfree shadows of sperms that are simultaneously acquired at two different wavelengths, emanating from two partially-coherent sources that are placed at 45° with respect to each other. This multiangle and multicolor illumination scheme permits us to dynamically track the 3D motion of human sperms across a field-of-view of > 17 mm(2) and depth-of-field of approximately 0.5-1 mm with submicron positioning accuracy. The large statistics provided by this lensfree imaging platform revealed that only approximately 4-5% of the motile human sperms swim along well-defined helices and that this percentage can be significantly suppressed under seminal plasma. Furthermore, among these observed helical human sperms, a significant majority (approximately 90%) preferred right-handed helices over left-handed ones, with a helix radius of approximately 0.5-3 μm, a helical rotation speed of approximately 3-20 rotations/s and a linear speed of approximately 20-100 μm/s. This high-throughput 3D imaging platform could in general be quite valuable for observing the statistical swimming patterns of various other microorganisms, leading to new insights in their 3D motion and the underlying biophysics.
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