ArticleInfection and immunity2012
Transposon mutagenesis of Salmonella enterica serovar Enteritidis identifies genes that contribute to invasiveness in human and chicken cells and survival in egg albumen.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
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- Salmonella Enteritidis T1SS protein SiiD inhibits NLRP3 inflammasome activation via repressing the mtROS-ASC dependent pathway.PLoS pathogens · 2023Article
- Genome-wide fitness analysis identifies genes required forMicrobial genomics · 2023Article
- Untangling Cellular Host-Pathogen Encounters at Infection Bottlenecks.Infection and immunity · 2023Review
- Egg-associatedFrontiers in microbiology · 2023Article
- Article
- Pathogenesis-Related Gene Expression in Response toFrontiers in veterinary science · 2022Article
- Infection Heterogeneity and Microbiota Differences in Chicks Infected byMicroorganisms · 2021Article
- Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Characterization of Basal Transcriptomes Identifies Potential Metabolic and Virulence-Associated Adaptations Among Diverse NontyphoidalFrontiers in microbiology · 2021Article
- Phylogenetic structure of Salmonella Enteritidis provides context for a foodborne outbreak in Peru.Scientific reports · 2020Article
- Refined functional carbohydrates reduce adhesion of Salmonella and Campylobacter to poultry epithelial cells in vitro.Poultry science · 2020Article
- Role ofmSphere · 2020Article
- The dietary combination of essential oils and organic acids reduces Salmonella enteritidis in challenged chicks.Poultry science · 2019Article
- Role of CpxR in Biofilm Development: Expression of Key Fimbrial, O-Antigen and Virulence Operons ofInternational journal of molecular sciences · 2019Article
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5 authors.
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Abstract
Salmonella enterica serovar Enteritidis is an important food-borne pathogen, and chickens are a primary reservoir of human infection. While most knowledge about Salmonella pathogenesis is based on research conducted on Salmonella enterica serovar Typhimurium, S. Enteritidis is known to have pathobiology specific to chickens that impacts epidemiology in humans. Therefore, more information is needed about S. Enteritidis pathobiology in comparison to that of S. Typhimurium. We used transposon mutagenesis to identify S. Enteritidis virulence genes by assay of invasiveness in human intestinal epithelial (Caco-2) cells and chicken liver (LMH) cells and survival within chicken (HD-11) macrophages as a surrogate marker for virulence. A total of 4,330 transposon insertion mutants of an invasive G1 Nal(r) strain were screened using Caco-2 cells. This led to the identification of attenuating mutations in a total of 33 different loci, many of which include genes previously known to contribute to enteric infection (e.g., Salmonella pathogenicity island 1 [SPI-1], SPI-4, SPI-5, CS54, fliH, fljB, csgB, spvR, and rfbMN) in S. Enteritidis and other Salmonella serovars. Several genes or genomic islands that have not been reported previously (e.g., SPI-14, ksgA, SEN0034, SEN2278, and SEN3503) or that are absent in S. Typhimurium or in most other Salmonella serovars (e.g., pegD, SEN1152, SEN1393, and SEN1966) were also identified. Most mutants with reduced Caco-2 cell invasiveness also showed significantly reduced invasiveness in chicken liver cells and impaired survival in chicken macrophages and in egg albumen. Consequently, these genes may play an important role during infection of the chicken host and also contribute to successful egg contamination by S. Enteritidis.
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