ArticleAntimicrobial agents and chemotherapy2012
Novel antibiofilm chemotherapy targets exopolysaccharide synthesis and stress tolerance in Streptococcus mutans to modulate virulence expression in vivo.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 70 citations in OpenAlex.
- Modulating Oral Microbiota to Prevent Dental Caries: A Microbial Ecology Approach.Dentistry journal · 2026Review
- Food-grade Lacticaseibacillus paracasei postbiotics suppress oral Streptococcus mutans biofilm formation and cariogenicity.NPJ science of food · 2026Article
- Effects of Berberine chloride hydrate on cariogenic multispecies streptococcal biofilm.Journal of oral microbiology · 2026Article
- Reproducible 3D bioprinting ofMicrobiology spectrum · 2025Article
- Effects of Chewing Gum on Plaque Index: A 3-Dimensional Colorimetric Analysis.Dentistry journal · 2025Article
- Synergistic action of specialized metabolites from divergent biosynthesis in the human oral microbiome.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Inter-kingdom interactions and environmental influences on the oral microbiome in severe early childhood caries.Microbiology spectrum · 2025Article
- Molecular insights into antibiofilm inhibitors of Streptococcus mutans glucosyltransferases through in silico approaches.Scientific reports · 2025Article
- Natural compounds: new therapeutic approach for inhibition ofFrontiers in pharmacology · 2025Review
- Anticariogenic activity of marine brown algaeFrontiers in cellular and infection microbiology · 2024Article
- Effect of Extracts, Fractions, and Isolated Molecules ofAntibiotics (Basel, Switzerland) · 2023Article
- Inhibition of biofilm formation and virulence factors of cariogenic oral pathogenJournal of oral microbiology · 2023Article
- Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Distinct Agents Induce Streptococcus mutans Cells with Altered Biofilm Formation Capacity.Microbiology spectrum · 2022Article
- Topical Application of 4'-Hydroxychalcone in Combination withACS omega · 2022Article
- Lactobacilli and human dental caries: more than mechanical retention.Microbiology (Reading, England) · 2022Article
- Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formationJournal of oral microbiology · 2022Article
- Compounds with Distinct Targets Present Diverse Antimicrobial and Antibiofilm Efficacy againstJournal of fungi (Basel, Switzerland) · 2021Article
- Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence.Dental materials : official publication of the Academy of Dental Materials · 2021Article
- Symbiosis and Dysbiosis of the Human Mycobiome.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Fluoride is the mainstay of dental caries prevention, and yet current applications offer incomplete protection and may not effectively address the infectious character of the disease. Therefore, we evaluated the effectiveness of a novel combination therapy (CT; 2 mM myricetin, 4 mM tt-farnesol, 250 ppm of fluoride) that supplements fluoride with naturally occurring, food-derived, antibiofilm compounds. Treatment regimens simulating those experienced clinically (twice daily for ≤60 s) were used both in vitro over a saliva-coated hydroxyapatite biofilm model and in vivo with a rodent model of dental caries. The effectiveness of CT was evaluated based on the incidence and severity of carious lesions (compared to fluoride or vehicle control). We found that CT was superior to fluoride (positive control, P < 0.05); topical applications dramatically reduced caries development in Sprague-Dawley rats, all without altering the Streptococcus mutans or total populations within the plaque. We subsequently identified the underlying mechanisms through which applications of CT modulate biofilm virulence. CT targets expression of key Streptococcus mutans genes during biofilm formation in vitro and in vivo. These are associated with exopolysaccharide matrix synthesis (gtfB) and the ability to tolerate exogenous stress (e.g., sloA), which are essential for cariogenic biofilm assembly. We also identified a unique gene (SMU.940) that was severely repressed and may represent a potentially novel target; its inactivation disrupted exopolysaccharide accumulation and matrix development. Altogether, CT may be clinically more effective than current anticaries modalities, targeting expression of bacterial virulence associated with pathogenesis of the disease. These observations may have relevance for development of enhanced therapies against other biofilm-dependent infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.