ArticleBMC microbiology2012
Single cell analysis of Vibrio harveyi uncovers functional heterogeneity in response to quorum sensing signals.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Psychrophilic Quorum Sensing Genes Enable Unimodal, Adjustable Protein Expression Across the EntireBiotech (Basel (Switzerland)) · 2026Article
- Single-cell phenotypic heterogeneity shapes quorum signaling dynamics in Pseudomonas aeruginosa.Nature communications · 2026Article
- Quorum sensing going wild.iScience · 2023Review
- Maximizing Growth Yield and Dispersal via Quorum Sensing Promotes Cooperation in Vibrio Bacteria.Applied and environmental microbiology · 2018Article
- Spatial determinants of quorum signaling in aProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Complete genome analysis of Gluconacetobacter xylinus CGMCC 2955 for elucidating bacterial cellulose biosynthesis and metabolic regulation.Scientific reports · 2018Article
- Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation bymBio · 2018Article
- The 9Frontiers in cellular and infection microbiology · 2018Article
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Quorum Sensing Gene Regulation by LuxR/HapR Master Regulators in Vibrios.Journal of bacteriology · 2017Review
- Article
- Specificity and complexity in bacterial quorum-sensing systems.FEMS microbiology reviews · 2016Review
- Parallel quorum sensing signaling pathways in Vibrio cholerae.Current genetics · 2016Review
- Phenotypic Heterogeneity, a Phenomenon That May Explain Why Quorum Sensing Does Not Always Result in Truly Homogenous Cell Behavior.Applied and environmental microbiology · 2015Review
- Core principles of bacterial autoinducer systems.Microbiology and molecular biology reviews : MMBR · 2015Article
- Quorum sensing triggers the stochastic escape of individual cells from Pseudomonas putida biofilms.Nature communications · 2015Article
- Evidence of autoinducer-dependent and -independent heterogeneous gene expression in Sinorhizobium fredii NGR234.Applied and environmental microbiology · 2014Article
- The CtrA phosphorelay integrates differentiation and communication in the marine alphaproteobacterium Dinoroseobacter shibae.BMC genomics · 2014Article
- Applications of flow cytometry to characterize bacterial physiological responses.BioMed research international · 2014Review
- Collective decision-making in microbes.Frontiers in microbiology · 2014Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio harveyi and closely related species are important pathogens in aquaculture. A complex quorum sensing cascade involving three autoinducers controls bioluminescence and several genes encoding virulence factors. Single cell analysis of a V. harveyi population has already indicated intercellular heterogeneity in the production of bioluminescence. This study was undertaken to analyze the expression of various autoinducer-dependent genes in individual cells.
resultsHere we used reporter strains bearing promoter::gfp fusions to monitor the induction/repression of three autoinducer-regulated genes in wild type conjugates at the single cell level. Two genes involved in pathogenesis - vhp and vscP, which code for an exoprotease and a component of the type III secretion system, respectively, and luxC (the first gene in the lux operon) were chosen for analysis. The lux operon and the exoprotease gene are induced, while vscP is repressed at high cell density. As controls luxS and recA, whose expression is not dependent on autoinducers, were examined. The responses of the promoter::gfp fusions in individual cells from the same culture ranged from no to high induction. Importantly, simultaneous analysis of two autoinducer induced phenotypes, bioluminescence (light detection) and exoproteolytic activity (fluorescence of a promoter::gfp fusion), in single cells provided evidence for functional heterogeneity within a V. harveyi population.
conclusionsAutoinducers are not only an indicator for cell density, but play a pivotal role in the coordination of physiological activities within the population.
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