ArticleZebrafish2012
An optimized method for delivering flow tracer particles to intravital fluid environments in the developing zebrafish.
Article in Zebrafish, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Biotin-Decorated Inulin-Based Polymeric Micelles Unveil Their Dual-Targeting Ability for the Potential Treatment of Glioblastoma Multiforme through theMolecular pharmaceutics · 2026Article
- Effects of Oligolysine-Polyethylene Glycol Coating on the Biodistribution of Wireframe DNA Origami Nanosheets in Zebrafish Embryos.ACS nano · 2025Article
- Zebrafish as a Versatile Model for Cardiovascular Research: Peering into the Heart of the Matter.Cells · 2025Review
- Brain penetration of peripheral extracellular vesicles from Alzheimer's patients and induction of microglia activation.Journal of extracellular biology · 2025Article
- Multivalent insulin receptor activation using insulin-DNA origami nanostructures.Nature nanotechnology · 2024Article
- Cardiac forces regulate zebrafish heart valve delamination by modulating Nfat signaling.PLoS biology · 2022Article
- SARS-CoV-2 spike protein causes blood coagulation and thrombosis by competitive binding to heparan sulfate.International journal of biological macromolecules · 2021Article
- Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos.Biomacromolecules · 2020Article
- Ultrabright fluorescent silica nanoparticles for in vivo targeting of xenografted human tumors and cancer cells in zebrafish.Nanoscale · 2019Article
- Deep learning image recognition enables efficient genome editing in zebrafish by automated injections.PloS one · 2019Article
- High-speed extended-volume blood flow measurement using engineered point-spread function.Biomedical optics express · 2018Article
- Functional and genetic analysis of choroid plexus development in zebrafish.Frontiers in neuroscience · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Growing evidence suggests that intravital flow-structure interactions are critical morphogens for normal embryonic development and disease progression, but fluid mechanical studies aimed at investigating these interactions have been limited in their ability to visualize and quantify fluid flow. In this study, we describe a protocol for injecting small (≤1.0 μm) tracer particles into fluid beds of the larval zebrafish to facilitate microscale fluid mechanical analyses. The microinjection apparatus and associated borosilicate pipette design, typically blunt-tipped with a 2-4 micron tip O.D., yielded highly linear (r(2)=0.99) in vitro bolus ejection volumes. The physical characteristics of the tracer particles were optimized for efficient particle delivery. Seeding densities suitable for quantitative blood flow mapping (≥50 thousand tracers per fish) were routinely achieved and had no adverse effects on zebrafish physiology or long-term survivorship. The data and methods reported here will prove valuable for a broad range of in vivo imaging technologies [e.g., particle-tracking velocimetry, μ-Doppler, digital particle image velocimetry (DPIV), and 4-dimensional-DPIV] which rely on tracer particles to visualize and quantify fluid flow in the developing zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.