ArticlePloS one2012
Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- The Role of Phytosterol Derivatives in Inhibiting LuxS-Mediated Quorum Sensing and Biofilm Formation in Vibrio parahaemolyticus.Molecular biotechnology · 2026Article
- The effect of bile salts stress on the biology and transcriptome characteristics of Vibrio parahaemolyticus under low salt environment.Archives of microbiology · 2026Article
- Functional divergence and regulatory network of the type VI secretion system inJournal of bacteriology · 2026Review
- Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis inFrontiers in microbiology · 2026Article
- The multifunctional type VI secretion system ofApplied and environmental microbiology · 2025Review
- Article
- AphA-dependent c-di-GMP production inJournal of bacteriology · 2025Article
- CalR is an activator of biofilm formation inApplied and environmental microbiology · 2025Article
- Investigating c-di-GMP Signaling in Vibrio parahaemolyticus: Biological Effects and Mechanisms of Regulation.Current microbiology · 2025Review
- The coordinated regulatory impact of AcsS and TpdA on biofilm formation inFrontiers in microbiology · 2025Article
- AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.Current microbiology · 2024Article
- ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 inGut microbes · 2023Article
- Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation.Current microbiology · 2023Article
- Quorum sensing and QsvR tightly control the transcription ofFrontiers in microbiology · 2023Article
- QsvR and OpaR coordinately repress biofilm formation byFrontiers in microbiology · 2023Article
- Regulatory Small RNA Qrr2 Is Expressed Independently of Sigma Factor-54 and Can Function as the Sole Qrr Small RNA To Control Quorum Sensing in Vibrio parahaemolyticus.Journal of bacteriology · 2022Article
- Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit inFrontiers in microbiology · 2021Article
- OpaR Controls the Metabolism of c-di-GMP inFrontiers in microbiology · 2021Article
- Direct Binding and Regulation by Fur and HapR of the Intermediate Regulator and Virulence Factor Genes Within the ToxR Virulence Regulon inFrontiers in microbiology · 2020Article
- Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAphA is the master quorum-sensing (QS) regulator operating at low cell density in vibrios. Molecular regulation of target genes by AphA has been characterized in Vibrio harveyi and V. cholerae, but it is still poorly understood in V. parahaemolyticus. METHODOLOGY/PRINCIPAL
findingsThe AphA proteins are extremely conserved in V. parahaemolyticus, Vibrio sp. Ex25, Vibrio sp. EJY3, V. harveyi, V. vulnificus, V. splendidus, V. anguillarum, V. cholerae, and V. furnissii. The above nine AphA orthologs appear to recognize conserved cis-acting DNA signals which can be represented by two consensus constructs, a 20 bp box sequence and a position frequency matrix. V. parahaemolyticus AphA represses the transcription of ahpA, qrr4, and opaR through direct AphA-target promoter DNA association, while it inhibits the qrr2-3 transcription in an indirect manner. Translation and transcription starts, core promoter elements for sigma factor recognition, Shine-Dalgarno sequences for ribosome recognition, and AphA-binding sites (containing corresponding AphA box-like sequences) were determined for the three direct AphA targets ahpA, qrr4, and opaR in V. parahaemolyticus. CONCLUSIONS/SIGNIFICANCE: AphA-mediated repression of ahpA, qrr2-4, and opaR was characterized in V. parahaemolyticus by using multiple biochemical and molecular experiments. The computational promoter analysis indicated the conserved mechanism of transcriptional regulation of QS regulator-encoding genes ahpA, qrr4, and opaR in vibrios.
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