ArticlePloS one2012
A large insertion in bHLH transcription factor BrTT8 resulting in yellow seed coat in Brassica rapa.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 132 citations in OpenAlex.
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- Transposable elements drive evolution and perturb gene expression in Brassica rapa and B. oleracea.The Plant journal : for cell and molecular biology · 2025Article
- Enhancing Oil Content in Oilseed Crops: Genetic Insights, Molecular Mechanisms, and Breeding Approaches.International journal of molecular sciences · 2025Review
- TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei.Plant molecular biology · 2025Article
- Natural variations inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A bHLH transcription factor RrUNE12 regulates salt tolerance and promotes ascorbate synthesis.Plant cell reports · 2025Article
- Comparative Transcriptomics Uncovers Upstream Factors RegulatingPlants (Basel, Switzerland) · 2024Article
- Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L.Plant biotechnology journal · 2024Article
- Identification of Yellow Seed Color Genes Using Bulked Segregant RNA Sequencing inInternational journal of molecular sciences · 2024Article
- Molecular evolution analysis of MYB5 in Brassicaceae with specific focus on seed coat color of Brassica napus.BMC plant biology · 2024Article
- Classical and machine learning tools for identifying yellow-seededFrontiers in genetics · 2024Article
- Article
- Interaction between phenylpropane metabolism and oil accumulation in the developing seed of Brassica napus revealed by high temporal-resolution transcriptomes.BMC biology · 2023Article
- Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.Nature communications · 2023Article
- Review
- Fine mapping of BnDM1-the gene regulating indeterminate inflorescence in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Transcriptome analysis of ovules offers early developmental clues after fertilization in3 Biotech · 2023Article
- QTL Mapping and Transcriptome Analysis Reveal Candidate Genes Regulating Seed Color inInternational journal of molecular sciences · 2023Article
- Genome-wide identification and expression analysis of the anthocyanin-related genes during seed coat development in six Brassica species.BMC genomics · 2023Article
12 more citing papers are in PubMed but not listed here.
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11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yellow seed is a desirable quality trait of the Brassica oilseed species. Previously, several seed coat color genes have been mapped in the Brassica species, but the molecular mechanism is still unknown. In the present investigation, map-based cloning method was used to identify a seed coat color gene, located on A9 in B. rapa. Blast analysis with the Arabidopsis genome showed that there were 22 Arabidopsis genes in this region including at4g09820 to at4g10620. Functional complementation test exhibited a phenotype reversion in the Arabidopsis thaliana tt8-1 mutant and yellow-seeded plant. These results suggested that the candidate gene was a homolog of TRANSPARENT TESTA8 (TT8) locus. BrTT8 regulated the accumulation of proanthocyanidins (PAs) in the seed coat. Sequence analysis of two alleles revealed a large insertion of a new class of transposable elements, Helitron in yellow sarson. In addition, no mRNA expression of BrTT8 was detected in the yellow-seeded line. It indicated that the natural transposon might have caused the loss in function of BrTT8. BrTT8 encodes a basic/helix-loop-helix (bHLH) protein that shares a high degree of similarity with other bHLH proteins in the Brassica. Further expression analysis also revealed that BrTT8 was involved in controlling the late biosynthetic genes (LBGs) of the flavonoid pathway. Our present findings provided with further studies could assist in understanding the molecular mechanism involved in seed coat color formation in Brassica species, which is an important oil yielding quality trait.
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