ArticleJournal of bacteriology2012
Analysis of the regulated transcriptome of Neisseria meningitidis in human blood using a tiling array.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Neisseria meningitidis Sibling Small Regulatory RNAs Connect Metabolism with Colonization by Controlling Propionate Use.Journal of bacteriology · 2023Article
- Development of a Sensitive Assay to Screen NanoparticlesACS biomaterials science & engineering · 2020Article
- Article
- Anticoagulants impact on innate immune responses and bacterial survival in whole blood models of Neisseria meningitidis infection.Scientific reports · 2018Article
- Neisseria genomics: current status and future perspectives.Pathogens and disease · 2017Review
- The primary transcriptome of Neisseria meningitidis and its interaction with the RNA chaperone Hfq.Nucleic acids research · 2017Article
- The Hfq regulon ofFEBS open bio · 2017Article
- Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence.BMC genomics · 2017Article
- Article
- Article
- Characterization of a novel antisense RNA in the major pilin locus of Neisseria meningitidis influencing antigenic variation.Journal of bacteriology · 2015Article
- Global transcriptome analysis reveals small RNAs affecting Neisseria meningitidis bacteremia.PloS one · 2015Article
- A large genomic island allows Neisseria meningitidis to utilize propionic acid, with implications for colonization of the human nasopharynx.Molecular microbiology · 2014Article
- Next-generation annotation of prokaryotic genomes with EuGene-P: application to Sinorhizobium meliloti 2011.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
- Peripheral blood RNA gene expression profiling in patients with bacterial meningitis.Frontiers in neuroscience · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neisseria meningitidis is the major cause of septicemia and meningococcal meningitis. During the course of infection, the bacterium must adapt to different host environments as a crucial factor for survival and dissemination; in particular, one of the crucial factors in N. meningitidis pathogenesis is the ability to grow and survive in human blood. We recently showed that N. meningitidis alters the expression of 30% of the open reading frames (ORFs) of the genome during incubation in human whole blood and suggested the presence of fine regulation at the gene expression level in order to control this step of pathogenesis. In this work, we used a customized tiling oligonucleotide microarray to define the changes in the whole transcriptional profile of N. meningitidis in a time course experiment of ex vivo bacteremia by incubating bacteria in human whole blood and then recovering RNA at different time points. The application of a newly developed bioinformatic tool to the tiling array data set allowed the identification of new transcripts--small intergenic RNAs, cis-encoded antisense RNAs, mRNAs with extended 5' and 3' untranslated regions (UTRs), and operons--differentially expressed in human blood. Here, we report a panel of expressed small RNAs, some of which can potentially regulate genes involved in bacterial metabolism, and we show, for the first time in N. meningitidis, extensive antisense transcription activity. This analysis suggests the presence of a circuit of regulatory RNA elements used by N. meningitidis to adapt to proliferate in human blood that is worthy of further investigation.
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