ArticleProceedings of the National Academy of Sciences of the United States of America2012
Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- In Vitro Spermatogenesis: How Far from Clinical Application?Current urology reports · 2016Pooled it
- Generation of functionally competent testicular somatic cells from pluripotent stem cells.Science advances · 2026Article
- In vitro system completely restores oogenesis in congenitally infertile mice.Human reproduction (Oxford, England) · 2025Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration.Clinical and experimental reproductive medicine · 2024Article
- Etiology of Male Infertility: an Update.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- Article
- Human in vitro spermatogenesis as a regenerative therapy - where do we stand?Nature reviews. Urology · 2023Review
- Novel discovery of schisandrin A regulating the interplay of autophagy and apoptosis in oligoasthenospermia by targeting SCF/c-kit and TRPV1Acta pharmaceutica Sinica. B · 2023Article
- Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source.Animals : an open access journal from MDPI · 2022Article
- Protein kinase A drives paracrine crisis and WNT4-dependent testis tumor in Carney complex.The Journal of clinical investigation · 2021Article
- Review
- Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility.Cells · 2020Review
- Sertoli cell ablation and replacement of the spermatogonial niche in mouse.Nature communications · 2020Article
- In vitro spermatogenesis: A century-long research journey, still half way around.Reproductive medicine and biology · 2018Review
- Progress and future prospect ofOncotarget · 2017Review
- Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System.Birth defects research · 2017Article
- Development of reproductive engineering techniques at the RIKEN BioResource Center.Experimental animals · 2017Review
- Targeted Disruption of miR-17-92 Impairs Mouse Spermatogenesis by Activating mTOR Signaling Pathway.Medicine · 2016Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Male infertility is most commonly caused by spermatogenic defects or insufficiencies, the majority of which are as yet cureless. Recently, we succeeded in cultivating mouse testicular tissues for producing fertile sperm from spermatogonial stem cells. Here, we show that one of the most severe types of spermatogenic defect mutant can be treated by the culture method without any genetic manipulations. The Sl/Sl(d) mouse is used as a model of such male infertility. The testis of the Sl/Sl(d) mouse has only primitive spermatogonia as germ cells, lacking any sign of spermatogenesis owing to mutations of the c-kit ligand (KITL) gene that cause the loss of membrane-bound-type KITL from the surface of Sertoli cells. To compensate for the deficit, we cultured testis tissues of Sl/Sl(d) mice with a medium containing recombinant KITL and found that it induced the differentiation of spermatogonia up to the end of meiosis. We further discovered that colony stimulating factor-1 (CSF-1) enhances the effect of KITL and promotes spermatogenesis up to the production of sperm. Microinsemination of haploid cells resulted in delivery of healthy offspring. This study demonstrated that spermatogenic impairments can be treated in vitro with the supplementation of certain factors or substances that are insufficient in the original testes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.