ArticlePlant physiology2012
Predictive modeling of biomass component tradeoffs in Brassica napus developing oilseeds based on in silico manipulation of storage metabolism.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- PII interactions with the acetyl-CoA carboxylase subunits BADC and BCCP co-regulate lipid and nitrogen metabolism in Arabidopsis.Plant physiology · 2025Article
- Comparative transcriptomic analysis provides insights into the genetic networks regulating oil differential production in oil crops.BMC biology · 2024Article
- Tandem Mass Tag-Based Quantitative Proteomics Reveals Implication of a Late Embryogenesis Abundant Protein (BnLEA57) in Seed Oil Accumulation inFrontiers in plant science · 2022Article
- Elucidation of Triacylglycerol Overproduction in the CFrontiers in plant science · 2022Article
- Review
- Plant cell cultures as heterologous bio-factories for secondary metabolite production.Plant communications · 2021Review
- Transcriptome integrated metabolic modeling of carbon assimilation underlying storage root development in cassava.Scientific reports · 2021Article
- Cellular Plasticity in Response to Suppression of Storage Proteins in theThe Plant cell · 2020Article
- 13C-Metabolic Flux Analysis in Developing Flax (Metabolites · 2019Article
- Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis.Scientific reports · 2019Article
- An Integrated Bioinformatics Analysis Reveals Divergent Evolutionary Pattern of Oil Biosynthesis in High- and Low-Oil Plants.PloS one · 2016Article
- Modeling Rice Metabolism: From Elucidating Environmental Effects on Cellular Phenotype to Guiding Crop Improvement.Frontiers in plant science · 2016Review
- Flux Balance Analysis of Plant Metabolism: The Effect of Biomass Composition and Model Structure on Model Predictions.Frontiers in plant science · 2016Article
- Inference and Prediction of Metabolic Network Fluxes.Plant physiology · 2015Article
- Oil biosynthesis in a basal angiosperm: transcriptome analysis of Persea Americana mesocarp.BMC plant biology · 2015Article
- Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.Plant physiology · 2015Article
- Improved evidence-based genome-scale metabolic models for maize leaf, embryo, and endosperm.Frontiers in plant science · 2015Article
- Multigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis.Plant physiology · 2014Article
- Integration of a constraint-based metabolic model of Brassica napus developing seeds with (13)C-metabolic flux analysis.Frontiers in plant science · 2014Article
- Transcript abundance on its own cannot be used to infer fluxes in central metabolism.Frontiers in plant science · 2014Article
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2 authors.
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Abstract
Seed oil content is a key agronomical trait, while the control of carbon allocation into different seed storage compounds is still poorly understood and hard to manipulate. Using bna572, a large-scale model of cellular metabolism in developing embryos of rapeseed (Brassica napus) oilseeds, we present an in silico approach for the analysis of carbon allocation into seed storage products. Optimal metabolic flux states were obtained by flux variability analysis based on minimization of the uptakes of substrates in the natural environment of the embryo. For a typical embryo biomass composition, flux sensitivities to changes in different storage components were derived. Upper and lower flux bounds of each reaction were categorized as oil or protein responsive. Among the most oil-responsive reactions were glycolytic reactions, while reactions related to mitochondrial ATP production were most protein responsive. To assess different biomass compositions, a tradeoff between the fractions of oil and protein was simulated. Based on flux-bound discontinuities and shadow prices along the tradeoff, three main metabolic phases with distinct pathway usage were identified. Transitions between the phases can be related to changing modes of the tricarboxylic acid cycle, reorganizing the usage of organic carbon and nitrogen sources for protein synthesis and acetyl-coenzyme A for cytosol-localized fatty acid elongation. The phase close to equal oil and protein fractions included an unexpected pathway bypassing α-ketoglutarate-oxidizing steps in the tricarboxylic acid cycle. The in vivo relevance of the findings is discussed based on literature on seed storage metabolism.
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