ArticleApplied and environmental microbiology2012
Phylogenetically novel LuxI/LuxR-type quorum sensing systems isolated using a metagenomic approach.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Quorum sensing in bacteria: in silico protein analysis, ecophysiology, and reconstruction of their evolutionary history.BMC genomics · 2024Article
- Article
- Functional metagenomic analysis of quorum sensing signaling in a nitrifying community.NPJ biofilms and microbiomes · 2021Article
- Strategies for Natural Products Discovery from Uncultured Microorganisms.Molecules (Basel, Switzerland) · 2021Review
- Metagenomic Insights Into the Microbial Assemblage Capable of Quorum Sensing and Quorum Quenching in Particulate Organic Matter in the Yellow Sea.Frontiers in microbiology · 2020Article
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Article
- Article
- Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes.Applied and environmental microbiology · 2017Article
- HqiA, a novel quorum-quenching enzyme which expands the AHL lactonase family.Scientific reports · 2017Article
- Computational Exploration of Putative LuxR Solos in Archaea and Their Functional Implications in Quorum Sensing.Frontiers in microbiology · 2017Article
- Genomic Analysis of Two Phylogenetically DistinctFrontiers in microbiology · 2017Article
- A Thermostable Bilirubin-Oxidizing Enzyme from Activated Sludge Isolated by a Metagenomic Approach.Microbes and environments · 2016Article
- Article
- Cloning and expression of quorum sensing N-acyl-homoserine synthase (LuxI) gene detected in Acinetobacter baumannii.Iranian journal of microbiology · 2016Article
- A New N-Acyl Homoserine Lactone Synthase in an Uncultured Symbiont of the Red Sea Sponge Theonella swinhoei.Applied and environmental microbiology · 2016Article
- Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Produces N-Acyl-Homoserine Lactone Autoinducers.Applied and environmental microbiology · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
- Can the natural diversity of quorum-sensing advance synthetic biology?Frontiers in bioengineering and biotechnology · 2015Review
- Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.Microbial biotechnology · 2015Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A great deal of research has been done to understand bacterial cell-to-cell signaling systems, but there is still a large gap in our current knowledge because the majority of microorganisms in natural environments do not have cultivated representatives. Metagenomics is one approach to identify novel quorum sensing (QS) systems from uncultured bacteria in environmental samples. In this study, fosmid metagenomic libraries were constructed from a forest soil and an activated sludge from a coke plant, and the target genes were detected using a green fluorescent protein (GFP)-based Escherichia coli biosensor strain whose fluorescence was screened by spectrophotometry. DNA sequence analysis revealed two pairs of new LuxI family N-acyl-L-homoserine lactone (AHL) synthases and LuxR family transcriptional regulators (clones N16 and N52, designated AubI/AubR and AusI/AusR, respectively). AubI and AusI each produced an identical AHL, N-dodecanoyl-L-homoserine lactone (C(12)-HSL), as determined by nuclear magnetic resonance (NMR) and mass spectrometry. Phylogenetic analysis based on amino acid sequences suggested that AusI/AusR was from an uncultured member of the Betaproteobacteria and AubI/AubR was very deeply branched from previously described LuxI/LuxR homologues in isolates of the Proteobacteria. The phylogenetic position of AubI/AubR indicates that they represent a QS system not acquired recently from the Proteobacteria by horizontal gene transfer but share a more ancient ancestry. We demonstrated that metagenomic screening is useful to provide further insight into the phylogenetic diversity of bacterial QS systems by describing two new LuxI/LuxR-type QS systems from uncultured bacteria.
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