ArticleNature methods2012
Reversible labeling of native and fusion-protein motifs.
Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Fluorometric Analysis of Carrier-Protein-Dependent Biosynthesis through a Conformationally Sensitive Solvatochromic Pantetheinamide Probe.ACS chemical biology · 2024Article
- Quantitative Characterization of Chain-Flipping of Acyl Carrier Protein ofJournal of the American Chemical Society · 2024Article
- BackboneBiomolecular NMR assignments · 2023Article
- Using NMR Titration Experiments to Study E. coli FAS-II- and AcpP-Mediated Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mechanism-based cross-linking probes capture the Escherichia coli ketosynthase FabB in conformationally distinct catalytic states.Acta crystallographica. Section D, Structural biology · 2022Article
- Enzymology of standalone elongating ketosynthases.Chemical science · 2022Review
- Characterization of Phosphopantetheinyl Hydrolase from Mycobacterium tuberculosis.Microbiology spectrum · 2021Article
- Two-Step Synthesis of α-Aryl-α-diazoamides as Modular Bioreversible Labels.Organic letters · 2021Article
- A Single Tool to Monitor Multiple Protein-Protein Interactions of theACS infectious diseases · 2019Article
- Article
- Molecular basis for interactions between an acyl carrier protein and a ketosynthase.Nature chemical biology · 2019Article
- Development of an effective protein-labeling system based on smart fluorogenic probes.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Review
- Recent progress in enzymatic protein labelling techniques and their applications.Chemical Society reviews · 2018Review
- Type II fatty acid and polyketide synthases: deciphering protein-protein and protein-substrate interactions.Natural product reports · 2018Review
- Matching Protein Interfaces for Improved Medium-Chain Fatty Acid Production.ACS synthetic biology · 2018Article
- PKS-NRPS Enzymology and Structural Biology: Considerations in Protein Production.Methods in enzymology · 2018Article
- In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A.Nature communications · 2016Article
- Probing the Substrate Specificity and Protein-Protein Interactions of the E. coli Fatty Acid Dehydratase, FabA.Chemistry & biology · 2015Article
- Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL.Journal of the American Chemical Society · 2015Article
- Using modern tools to probe the structure-function relationship of fatty acid synthases.Chembiochem : a European journal of chemical biology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The reversible covalent attachment of chemical probes to proteins has long been sought as a means to visualize and manipulate proteins. Here we demonstrate the full reversibility of post-translational custom pantetheine modification of Escherichia coli acyl carrier protein for visualization and functional studies. We use this iterative enzymatic methodology in vitro to reversibly label acyl carrier protein variants and apply these tools to NMR structural studies of protein-substrate interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.