ArticleThe Journal of steroid biochemistry and molecular biology2013
Histone deacetylation mediates the rejuvenation of osteoblastogenesis by the combination of 25(OH)D3 and parathyroid hormone in MSCs from elders.
Article in The Journal of steroid biochemistry and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Multiomics profiling reveals VDR as a central regulator of mesenchymal stem cell senescence with a known association with osteoporosis after high-fat diet exposure.International journal of oral science · 2024Article
- Identification of a clinical signature predictive of differentiation fate of human bone marrow stromal cells.Stem cell research & therapy · 2021Article
- Vitamin D Metabolism Revised: Fall of Dogmas.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Review
- Physiologic and pathophysiologic roles of extra renal CYP27b1: Case report and review.Bone reports · 2018Article
- Chronic kidney disease and vitamin D metabolism in human bone marrow-derived MSCs.Annals of the New York Academy of Sciences · 2017Review
- (+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor.Experimental and therapeutic medicine · 2017Article
- MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR.American journal of translational research · 2017Article
- Paracrine effects of haematopoietic cells on human mesenchymal stem cells.Scientific reports · 2015Article
- Epigenetic pathways regulating bone homeostasis: potential targeting for intervention of skeletal disorders.Current osteoporosis reports · 2014Review
- Vitamin D and gene networks in human osteoblasts.Frontiers in physiology · 2014Review
- Vitamin D endocrine system and osteoblasts.BoneKEy reports · 2014Article
- Vitamin D metabolism in human bone marrow stromal (mesenchymal stem) cells.Metabolism: clinical and experimental · 2013Review
- Functional impairment of bone formation in the pathogenesis of osteoporosis: the bone marrow regenerative competence.Current osteoporosis reports · 2013Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Vitamin D metabolites are important effectors of bone and mineral homeostasis. Human bone marrow stromal cells (hMSCs) are targets of 1α,25-dihydroxyvitamin D [1α,25(OH)2D] action to promote their differentiation to osteoblasts. Osteoblastogenesis is also stimulated by 25-hydroxyvitamin D [25(OH)D], an effect that requires conversion to 1α,25(OH)2D3 by 25-hydroxyvitamin D3 1α-hydroxylase (CYP27B1). These findings support an autocrine/paracrine role of vitamin D metabolism in osteoblastogenesis of hMSCs. In this study, we assessed whether and by what mechanisms osteoblastogenesis could be rejuvenated with hMSCs from elders. First, knockdown studies with VDR-siRNA showed that both the pro-differentiation and anti-proliferative effects of 1α,25(OH)2D3 required VDR. Second, 100nM 25(OH)D3 (p<0.01 vs. control, ANOVA) and 100nM PTH1-34 (p<0.05) significantly stimulated alkaline phosphatase (ALP) activity (a measure of osteoblastogenesis), with a synergistic effect when combined (p<0.001). Scriptaid, an inhibitor of histone deacetylase, blocked the effect of 25(OH)D3 and PTH on osteoblastogenesis. Scriptaid alone downregulated VDR in hMSCs. These data demonstrate that histone deacetylation is required for the synergistic effect of 25(OH)D3 and PTH on osteoblastogenesis in hMSCs. Both VDR siRNA and Scriptaid dowregulated VDR mRNA and inhibited osteoblastogenesis. Thus, epigenetic regulation of the VDR may be central to rejuvenating osteoblastogenesis in hMSCs from elders. This article is part of a Special Issue entitled 'Vitamin D Workshop'.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.