ArticleJournal of virological methods2013
Evaluation of the Tetracore Orthopox BioThreat® antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens.
Article in Journal of virological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Emerging strategies for monkeypox: antigen and antibody applications in diagnostics, vaccines, and treatments.Military Medical Research · 2025Review
- Diagnostic Accuracy of 3 Mpox Lateral Flow Assays for Antigen Detection, Democratic Republic of the Congo and United Kingdom.Emerging infectious diseases · 2025Article
- Detection of mpox and other orthopoxviruses using a lateral flow device as a point-of-care diagnostic.Microbiology spectrum · 2025Article
- Monkeypox: A Viral Zoonotic Disease of Rising Global Concern.Infectious diseases & immunity · 2024Review
- Biological Characteristics and Pathogenesis of Monkeypox Virus: An Overview.Advances in experimental medicine and biology · 2024Review
- Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids.Nano letters · 2023Article
- Human Monkeypox: A Comprehensive Overview of Epidemiology, Pathogenesis, Diagnosis, Treatment, and Prevention Strategies.Pathogens (Basel, Switzerland) · 2023Review
- Clinical and laboratory diagnosis of monkeypox (mpox): Current status and future directions.iScience · 2023Review
- Comprehensive overview of human monkeypox: epidemiology, clinical features, pathogenesis, diagnosis and prevention.Annals of medicine and surgery (2012) · 2023Article
- Internal Validation of a Real-Time qPCR Kit following the UNE/EN ISO/IEC 17025:2005 for Detection of the Re-EmergingDiagnostics (Basel, Switzerland) · 2023Article
- Monkeypox (mpox) virus: Classification, origin, transmission, genome organization, antiviral drugs, and molecular diagnosis.Journal of infection and public health · 2023Review
- Laboratory Diagnosis for Monkeypox: Clinical Practice and Important Points to Be Recognized.Advances in experimental medicine and biology · 2023Article
- Monkeypox Virus Infections in Humans.Clinical microbiology reviews · 2022Review
- Article
- Monkeypox: An Unfamiliar Virus-Clinical and Epidemiological Characteristics, Diagnosis, and Treatment with Special Emphasis on Oral Health.Diagnostics (Basel, Switzerland) · 2022Review
- Current and Perspective Sensing Methods for Monkeypox Virus.Bioengineering (Basel, Switzerland) · 2022Review
- Human monkeypox: A review of the literature.PLoS pathogens · 2022Review
- Monkeypox: The Re-emerging Terror.Cureus · 2022Review
- Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies.Trends in analytical chemistry : TRAC · 2021Review
- Emergence of Monkeypox as the Most Important Orthopoxvirus Infection in Humans.Frontiers in public health · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The commercially available Orthopox BioThreat® Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat® Alert assay reproducibly detected samples at concentrations of 10(7)pfu/ml for VACV and MPXV and positively identified samples containing 10(6)pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat® Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.