ArticleCurrent biology : CB2012
A genome-wide functional screen shows MAGI-1 is an L1CAM-dependent stabilizer of apical junctions in C. elegans.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 49 citations in OpenAlex.
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- Glial adherens junction proteins act with SAX-7/L1CAM to promote dendrite extension in C. elegans.Development (Cambridge, England) · 2026Article
- The tumor suppressor RASSF8: A WAVE interaction partner controlling migration and cohesion of invasive border cells inProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- C. elegans Afadin is required for epidermal morphogenesis and functionally interfaces with the cadherin-catenin complex and RhoGAP PAC-1/ARHGAP21.Developmental biology · 2024Article
- Tight junctions: from molecules to gastrointestinal diseases.Tissue barriers · 2023Article
- Zyxin contributes to coupling between cell junctions and contractile actomyosin networks during apical constriction.PLoS genetics · 2023Article
- SRGP-1/srGAP and AFD-1/afadin stabilize HMP-1/⍺-catenin at rosettes to seal internalization sites following gastrulation in C. elegans.PLoS genetics · 2023Article
- TES-1/Tes and ZYX-1/Zyxin protect junctional actin networks under tension during epidermal morphogenesis in the C. elegans embryo.Current biology : CB · 2022Article
- C. elegans srGAP is an α-catenin M domain-binding protein that strengthens cadherin-dependent adhesion during morphogenesis.Development (Cambridge, England) · 2022Article
- Cell polarity signalling at the birth of multicellularity: What can we learn from the first animals.Frontiers in cell and developmental biology · 2022Review
- MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis.Communications biology · 2021Article
- MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.Scientific reports · 2021Article
- Article
- Cdc42 regulates junctional actin but not cell polarization in theThe Journal of cell biology · 2017Article
- Defining functional interactions during biogenesis of epithelial junctions.Nature communications · 2016Article
- Developmentally programmed germ cell remodelling by endodermal cell cannibalism.Nature cell biology · 2016Article
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundIn multicellular organisms, cell-cell junctions are involved in many aspects of tissue morphogenesis. α-catenin links the cadherin-catenin complex (CCC) to the actin cytoskeleton, stabilizing cadherin-dependent adhesions.
resultsTo identify modulators of cadherin-based cell adhesion, we conducted a genome-wide RNAi screen in C. elegans and uncovered MAGI-1, a highly conserved protein scaffold. Loss of magi-1 function in wild-type embryos results in disorganized epithelial migration and occasional morphogenetic failure. MAGI-1 physically interacts with the putative actin regulator AFD-1/afadin; knocking down magi-1 or afd-1 function in a hypomorphic α-catenin background leads to complete morphogenetic failure and actin disorganization in the embryonic epidermis. MAGI-1 and AFD-1 localize to a unique domain in the apical junction and normal accumulation of MAGI-1 at junctions requires SAX-7/L1CAM, which can bind MAGI-1 via its C terminus. Depletion of MAGI-1 leads to loss of spatial segregation and expansion of apical junctional domains and greater mobility of junctional proteins.
conclusionsOur screen is the first genome-wide approach to identify proteins that function synergistically with the CCC during epidermal morphogenesis in a living embryo. We demonstrate novel physical interactions between MAGI-1, AFD-1/afadin, and SAX-7/L1CAM, which are part of a functional interactome that includes components of the core CCC. Our results further suggest that MAGI-1 helps to partition and maintain a stable, spatially ordered apical junction during morphogenesis.
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