ArticleMicrobial cell factories2012
Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Posttranslational Targeting of a Recombinant Protein Promotes Its Efficient Secretion into the Escherichia coli Periplasm.Applied and environmental microbiology · 2019Article
- Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress.Applied and environmental microbiology · 2018Article
- Directed evolution to improve protein folding in vivo.Current opinion in structural biology · 2018Review
- The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli.Microbial cell factories · 2017Article
- Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs.Microbial cell factories · 2015Review
- Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli.Microbial cell factories · 2015Article
- Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein.Molecular biology reports · 2015Article
- Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies.Microbial cell factories · 2014Article
- High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli.Journal of visualized experiments : JoVE · 2014Article
- Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins.Microbial cell factories · 2013Review
- Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels.Microbial cell factories · 2013Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of heterologous disulfide bonded proteins in bacteria remains a biotechnological challenge. A rapid literature survey results in the identification of some interesting proposals, such as the option of producing functional proteins in the cytoplasm in the presence of sulfhydryl oxidases and isomerases. Furthermore, an ever-increasing number of applications refers to recombinant proteins displayed at the bacterial surface. Time will tell whether these developments will lead to universally accepted laboratory protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.